PXD017988

PXD017988 is an original dataset announced via ProteomeXchange.

Title	Identification of interactors of Sth1 in Candida albicans
Description	Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. To precisely determine the composition of the CaRSC complex, we immunopurified Myc-tagged Sth1, the core catalytic subunit of the CaRSC complex encoded by C3_02490C and identified its physical interactors using high-resolution tandem mass spectrometry (LC-MS/MS). Wild-type cells expressing untagged Sth1 served as negative control, and all immunoprecipitation experiments were analyzed in biological triplicates. No antibody controls for immunopurification were also analysed.
HostingRepository	PRIDE
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2020-11-16_07:56:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinutha K B
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 03:26:22	ID requested
⏵ 1	2020-11-16 07:56:10	announced

Balachandra VK, Verma J, Shankar M, Tucey TM, Traven A, Schittenhelm RB, Ghosh SK, The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits. PLoS Genet, 16(11):e1009071(2020) [pubmed]

submitter keyword: AP-MS, RSC, Candida albicans, Sth1

Santanu Kumar Ghosh
contact affiliation	Chromosome and Plasmid Segregation Lab, Department of Biosciences and Bioengineering, IIT Bombay, Mumbai, India
contact email	santanughosh@iitb.ac.in
lab head
Vinutha K B
contact affiliation	IIT Bombay and Monash University
contact email	vinutha.kb@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017988
     1. Label: PRIDE project
     2. Name: Identification of interactors of Sth1 in Candida albicans