PXD017987

PXD017987 is an original dataset announced via ProteomeXchange.

Title	Validation of Nri1/Nri2 physical interaction with the Candida albicans RSC complex
Description	Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. In this study, we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans (CaRSC). To precisely determine the composition of the CaRSC complex, we immunopurified Myc-tagged Sth1, the core catalytic subunit of the CaRSC complex encoded by C3_02490C and identified its physical interactors using high-resolution tandem mass spectrometry (LC-MS/MS). Strikingly, we identified two novel hitherto uncharacterized proteins that co-purified with Sth1, C2_01370p (Nri1) and C1_01800p (Nri2), whose homologs are annotated only in the CTG clade of fungi. Here, we performed reciprocal co-immunoprecipitations of Myc-tagged Nri1 and Nri2 followed by LC-MS/MS to validate these interactions. For each condition, biological duplicates were analyzed.
HostingRepository	PRIDE
AnnounceDate	2020-11-11
AnnouncementXML	Submission_2020-11-11_09:16:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinutha K B
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-11 03:26:18	ID requested
⏵ 1	2020-11-11 09:16:22	announced

Balachandra VK, Verma J, Shankar M, Tucey TM, Traven A, Schittenhelm RB, Ghosh SK, The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits. PLoS Genet, 16(11):e1009071(2020) [pubmed]

submitter keyword: AP-MS, Nri1, Nri2, Candida albicans, RSC

Santanu Kumar Ghosh
contact affiliation	Chromosome and Plasmid Segregation Lab, Department of Biosciences and Bioengineering, IIT Bombay, Mumbai, India
contact email	santanughosh@iitb.ac.in
lab head
Vinutha K B
contact affiliation	IIT Bombay and Monash University
contact email	vinutha.kb@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD017987

PRIDE project URI

• PRIDE
  1. PXD017987
     1. Label: PRIDE project
     2. Name: Validation of Nri1/Nri2 physical interaction with the Candida albicans RSC complex