PXD017963

PXD017963 is an original dataset announced via ProteomeXchange.

Title	Deciphering the protein secretome of leukocyte-platelet rich fibrin
Description	Leukocyte-platelet rich fibrin (L-PRF) is extensively used in the dentistry field and other clinical scenarios due to its regeneration properties. We obtained L-PRF membranes in absence of anticoagulants and cultured in DMEM. The secretomewas collected at days 3, 7 and 21 in order to identify the proteins secreted and the differences over time. Protein secretomeat day 3 was analysed by LC-MS/MS and differences in the proteome were analysed by Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH). Overall, 705 proteins were identified in the secretome of L-PRF membranes after 3 days of culture, including growth factors (EGF, PDGFA) and proteins related to platelet and neutrophil degranulation. A total of 202 differentially secreted proteins were quantified by SWATH when comparing secretomes at days 3, 7 and 21. The majority of them were enriched at day 3 such as MMP9, TSP1 and CO3. On the contrary, fibrinogen and CATS were found down-regulated at day 3. Growth factor and western blotting analysis corroborated the proteomic results. This is the most detailed proteome analysis of the L-PRF secretome to date. Proteins and growth factors identified, and their kinetics, provide novel information to further understand the wound healing properties of L-PRF.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:13:33.402.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-10 02:36:05	ID requested
⏵ 1	2021-09-09 00:13:34	announced

Hermida-Nogueira L, Barrachina MN, Mor, á, n LA, Bravo S, Diz P, Garc, í, a Á, Blanco J, Deciphering the secretome of leukocyte-platelet rich fibrin: towards a better understanding of its wound healing properties. Sci Rep, 10(1):14571(2020) [pubmed]

submitter keyword: Leukocyte-platelet rich fibrin (L-PRF), secretome, wound healing, SWATH

Susana B Bravo Lopez
contact affiliation	Proteomics Unit, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017963
     1. Label: PRIDE project
     2. Name: Deciphering the protein secretome of leukocyte-platelet rich fibrin