PXD017953

PXD017953 is an original dataset announced via ProteomeXchange.

Title	The Brassica Mature Pollen and Stigma Proteomes: Preparing to Meet
Description	Successful pollination brings together the mature pollen grain and stigma papilla to initiate an intricate series of molecular processes meant to eventually enable sperm cell delivery for fertilization and reproduction. At maturity, the pollen and stigma cells have acquired proteomes comprising the primary molecular effectors required upon their meeting. In Brassica species, knowledge of the roles and global composition of these proteomes is largely lacking. To address this gap, gel-free shotgun proteomics was performed on the mature pollen and stigma of Brassica carinata, a representative of the Brassica family and its many crop species (e.g. B. napus, B. oleracea, B. rapa), which holds considerable potential as a bio-industrial crop. 5608 and 7703 B. carinata mature pollen and stigma proteins were identified, respectively. The pollen and stigma proteomes were found to reflect not only their many common functional and developmental objectives, but also important differences underlying their cellular specialization. Isobaric tag for relative and absolute quantification (iTRAQ) was exploited in the first analysis of a developing Brassicaceae stigma, and uncovered 251 B. carinata proteins that were differentially abundant during stigma maturation, providing insight into proteins involved in the initial phases of pollination.
HostingRepository	PRIDE
AnnounceDate	2021-03-15
AnnouncementXML	Submission_2021-03-14_23:02:58.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renee Robinson
SpeciesList	scientific name: Brassica carinata; NCBI TaxID: 52824;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; dehydrated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-09 10:24:10	ID requested
1	2021-03-13 09:40:19	announced
⏵ 2	2021-03-14 23:02:59	announced	2021-03-15: Updated project metadata.

Robinson R, Sollapura V, Couroux P, Sprott D, Ravensdale M, Routly E, Xing T, Robert LS, The Brassica mature pollen and stigma proteomes: preparing to meet. Plant J, 107(5):1546-1568(2021) [pubmed]

10.1111/TPJ.15219;

submitter keyword: Brassica carinata, pollen, stigma, pollen-stigma interactions, pollen germination, pollen tube growth, proteomics, mass spectrometry, iTRAQ, LC-MS/MS

Dr. Laurian Robert, Dr. Renee Robinson
contact affiliation	Agriculture and Agri-Food Canada, Canada
contact email	drreneerobinson@gmail.com
lab head
Renee Robinson
contact affiliation	Agriculture and Agri-Food Canada, Carleton University
contact email	drreneerobinson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD017953

PRIDE project URI

• PRIDE
  1. PXD017953
     1. Label: PRIDE project
     2. Name: The Brassica Mature Pollen and Stigma Proteomes: Preparing to Meet