PXD017934

PXD017934 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Campylobacter jejuni grown in deoxycholate and following gene deletion
Description	Campylobacter jejuni is a major cause of food-borne gastroenteritis. Proteomics by label-based two-dimensional liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identified proteins associated with growth in 0.1% sodium deoxycholate (DOC, a component of gut bile salts), and system-wide validation was performed by data-independent acquisition (DIA-SWATH-MS). Proteins involved in nutrient transport were altered by DOC and aligned with intracellular changes to their respective carbon sources. DOC increased intracellular levels of sulfur-containing amino acids (cysteine and methionine) and the dipeptide cystine (Cys-Cys). A DOC induced transport protein was Cj0025c, which has sequence similarity to bacterial Cys-Cys transporters. Deletion of cj0025c (Δcj0025c) resulted in proteome changes consistent with sulfur starvation.
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-05-15_03:57:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017934
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lok Man
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationList	TMT6plex; Oxidation; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-09 03:16:02	ID requested
⏵ 1	2020-05-15 03:57:21	announced

Man L, Dale AL, Klare WP, Cain JA, Sumer-Bayraktar Z, Niewold P, Solis N, Cordwell SJ, Growth in Deoxycholate Reveals Cj0025c as a Cystine Transport Protein Required for Wild-type Human Infection Phenotypes. Mol Cell Proteomics, 19(8):1263-1280(2020) [pubmed]

submitter keyword: Campylobacter, pathogenesis, sulfur, nutrient uptake, proteomics

Stuart James Cordwell
contact affiliation	The University of Sydney
contact email	stuart.cordwell@sydney.edu.au
lab head
Lok Man
contact affiliation	The University of Sydney
contact email	lman5062@uni.sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017934
     1. Label: PRIDE project
     2. Name: Proteomics of Campylobacter jejuni grown in deoxycholate and following gene deletion