PXD017934 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of Campylobacter jejuni grown in deoxycholate and following gene deletion |
Description | Campylobacter jejuni is a major cause of food-borne gastroenteritis. Proteomics by label-based two-dimensional liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) identified proteins associated with growth in 0.1% sodium deoxycholate (DOC, a component of gut bile salts), and system-wide validation was performed by data-independent acquisition (DIA-SWATH-MS). Proteins involved in nutrient transport were altered by DOC and aligned with intracellular changes to their respective carbon sources. DOC increased intracellular levels of sulfur-containing amino acids (cysteine and methionine) and the dipeptide cystine (Cys-Cys). A DOC induced transport protein was Cj0025c, which has sequence similarity to bacterial Cys-Cys transporters. Deletion of cj0025c (Δcj0025c) resulted in proteome changes consistent with sulfur starvation. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-15 |
AnnouncementXML | Submission_2020-05-15_03:57:20.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD017934 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Lok Man |
SpeciesList | scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222; |
ModificationList | TMT6plex; Oxidation; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-03-09 03:16:02 | ID requested | |
⏵ 1 | 2020-05-15 03:57:21 | announced | |
Publication List
Man L, Dale AL, Klare WP, Cain JA, Sumer-Bayraktar Z, Niewold P, Solis N, Cordwell SJ, Growth in Deoxycholate Reveals Cj0025c as a Cystine Transport Protein Required for Wild-type Human Infection Phenotypes. Mol Cell Proteomics, 19(8):1263-1280(2020) [pubmed] |
Keyword List
submitter keyword: Campylobacter, pathogenesis, sulfur, nutrient uptake, proteomics |
Contact List
Stuart James Cordwell |
contact affiliation | The University of Sydney |
contact email | stuart.cordwell@sydney.edu.au |
lab head | |
Lok Man |
contact affiliation | The University of Sydney |
contact email | lman5062@uni.sydney.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017934 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017934
- Label: PRIDE project
- Name: Proteomics of Campylobacter jejuni grown in deoxycholate and following gene deletion