PXD017926
PXD017926 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Molecular remodeling in Populus PdKOR RNAi roots captured using LC-MS/MS proteomics |
| Description | Plants endo-beta-1,4-glucanases, belonging to Glycoside Hydrolase Family 9, have functional roles in cell wall biosynthesis and remodeling via endohydrolysis of (1-4)-beta-D-glucosidic linkages. Modification of cell wall chemistry via RNAi-mediated downregulation of PdKOR1, a endo-beta-1,4-glucanase gene, in Populus deltoides, has been shown to have functional consequences to the composition of secondary metabolome and ability of modified roots to interact with microbes. The molecular remodeling that underlies the observable differences at metabolic, physiological and morphological levels in roots is not well understood. Here we used a LC-MS/MS-based proteome profiling approach to survey the molecular remodeling in root tissues of PdKOR and control plants. A total of 14316 peptides were identified and these mapped to 7139 P. deltoides. Based on 90% sequence identity, the measured protein accessions represent 1187 functional protein groups. Analysis of GO categories and specific individual proteins shows differential expression of proteins relevant to plant-microbe interactions, cell wall chemistry and metabolism. This proteome dataset can serve as a useful resource for deriving new hypotheses and empirical testing pertaining to functional roles of proteins and pathways in differential priming of plant roots to interactions with microbes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-09-22 |
| AnnouncementXML | Submission_2020-09-22_07:06:04.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Paul Abraham |
| SpeciesList | scientific name: Populus deltoides; NCBI TaxID: 3696; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-03-06 11:34:24 | ID requested | |
| ⏵ 1 | 2020-09-22 07:06:04 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Populus, root tissue, LC-MS/MS |
Contact List
| Udaya Kalluri | |
|---|---|
| contact affiliation | ORNL |
| contact email | kalluriudayc@ornl.gov |
| lab head | |
| Paul Abraham | |
| contact affiliation | ORNL |
| contact email | pav@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085068/ |
to receive all new ProteomeXchange dataset release announcements!
