PXD017922

PXD017922 is an original dataset announced via ProteomeXchange.

Title	Trans-splicing of class I HLA bound peptides diversifies the immunopeptidome
Description	The diversity of peptides displayed by class I HLA plays an essential role in T cell immunity. The peptide repertoire is extended by various post-translational modifications, including cis-splicing of peptides from the same protein. Here, we have applied a novel bioinformatic workflow and demonstrate that spliced-peptides are also generated through trans-splicing (fusion of peptide segments from distinct antigens) and their abundance challenges current models of proteasomal-splicing that predict cis-splicing as the most probable outcome. These trans-spliced peptides display canonical HLA binding motif sequence features. These results highlight the unanticipated diversity of the immunopeptidome and have important implications for autoimmunity, vaccine design and immunotherapy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:34.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pouya Faridi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	TripleTOF 5600; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-06 01:30:00	ID requested
1	2021-12-05 23:43:00	announced
⏵ 2	2023-11-14 08:50:36	announced	2023-11-14: Updated project metadata.

Faridi P, Li C, Ramarathinam SH, Vivian JP, Illing PT, Mifsud NA, Ayala R, Song J, Gearing LJ, Hertzog PJ, Ternette N, Rossjohn J, Croft NP, Purcell AW, A subset of HLA-I peptides are not genomically templated: Evidence for cis- and trans-spliced peptide ligands. Sci Immunol, 3(28):(2018) [pubmed]

submitter keyword: HLA, post-translational modification, mass spectrometry,Immunopeptidome, proteasome, spliced peptides

Anthony Purcell
contact affiliation	Deputy Head (Research), Department of Biochemistry and Molecular Biology Head Immunoproteomics Laboratory Monash University, Clayton Campus Clayton 3800 Victoria Australia
contact email	anthony.purcell@monash.edu
lab head
Pouya Faridi
contact affiliation	Monash University
contact email	pouya.faridi@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD017922

PRIDE project URI

• PRIDE
  1. PXD017922
     1. Label: PRIDE project
     2. Name: Trans-splicing of class I HLA bound peptides diversifies the immunopeptidome