PXD017917

PXD017917 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of BipA in E. coli reveals the plasticity of 50S ribosome assembly
Description	BipA is a conserved translational GTPase that resembles elongation factor EF-G and 30S assembly factor LepA. Recent evidence suggests that BipA functions in 50S subunit assembly, but the precise role of the factor remains unclear. Here, we use stable isotope labeling of amino acids in culture and mass spectrometry (SILAC / MS) to examine the function of BipA in ribosome biogenesis. During growth at suboptimal temperature, loss of BipA leads to accumulation of immature large subunit particles (~40S) that lack several proteins. These include L2, L7/12, L10, L14, L16, L17, L19, L27, L28 and L32. Parallel analysis of the control (wild-type) strain shows accumulation of virtually identical intermediate particles, although at much lower levels. Further analysis showed that the main path of 50S assembly differs depending on media in which the cells are grown, demonstrating the robust and flexible nature of the assembly process.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:04.027.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-05 23:50:13	ID requested
1	2021-09-09 07:40:33	announced
⏵ 2	2024-10-22 05:29:04	announced	2024-10-22: Updated project metadata.

10.1016/j.jmb.2020.07.013;

Gibbs MR, Moon KM, Warner BR, Chen M, Bundschuh R, Foster LJ, Fredrick K, Functional Analysis of BipA in E. coli Reveals the Natural Plasticity of 50S Subunit Assembly. J Mol Biol, 432(19):5259-5272(2020) [pubmed]

submitter keyword: YchF, GTPase,protein synthesis, TypA, HflX

Kurt L. Fredrick
contact affiliation	Department of Microbiology and Center for RNA Biology, The Ohio State University, Columbus, OH 43210
contact email	Fredrick.5@osu.edu
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD017917

PRIDE project URI

• PRIDE
  1. PXD017917
     1. Label: PRIDE project
     2. Name: Functional analysis of BipA in E. coli reveals the plasticity of 50S ribosome assembly