PXD017903

PXD017903 is an original dataset announced via ProteomeXchange.

Title	The small protein CdrS is involved in cell division regulation
Description	Recently the function and cellular importance of small proteins became more and more visible. Whereas many examples of small protein functions have been described in eukarya and bacteria, less is known about small protein functions in archaea. A previous determination of the proteome in the halophilic archaeon Haloferax volcanii by mass spectrometry confirmed the expression of several annotated genes for small proteins. One such protein is encoded by HVO_0582. To identify the biological function of the encoded small protein we attempted to generate a deletion strain. Since we were not successful in generating the deletion we repressed HVO_0582 using CRISPRi. The repression strain showed a severe growth defect as well as strong changes in morphology. ChIPSeq experiments revealed several binding sites for this putative transcription factor. Transcriptome and proteome analyses show a clear effect on RNA and protein expression in the CRISPRi strain. Based on our findings we conclude that the small protein CdrS is involved in regulation of cell division / cell morphology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:24:28.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-05 05:49:36	ID requested
1	2021-08-09 02:17:19	announced
2	2021-08-09 02:37:00	announced	2021-08-09: Updated project metadata.
⏵ 3	2024-10-22 05:24:29	announced	2024-10-22: Updated project metadata.

Liao Y, Vogel V, Hauber S, Bartel J, Alkhnbashi OS, Maa, ß S, Schwarz TS, Backofen R, Becher D, Duggin IG, Marchfelder A, CdrS Is a Global Transcriptional Regulator Influencing Cell Division in Haloferax volcanii. mBio, 12(4):e0141621(2021) [pubmed]

10.1128/mbio.01416-21;

submitter keyword: cytosol, LC-MS, cell division, cell morphology, archaea, transcriptomics, CRISPRi, membrane,small proteins

Dörte Becher
contact affiliation	Department of Microbial Proteomics, Institute of Microbiology, Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017903
     1. Label: PRIDE project
     2. Name: The small protein CdrS is involved in cell division regulation