PXD017901

PXD017901 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics of different truffle species
Description	Truffles of the Tuber species as expensive foods are predestined for food fraud. To find markers for distinguishing different truffle species the proteomes as phenotype determining basis were investigated. First, established sample preparation protocols subsequent to LC-MS/MS analysis for a bottom up proteomic approach were tested on a truffle sample. The sample preparation included protein extraction and in-solution tryptic digestion. For protein extraction sodium deoxycholate (SDC), sodium dodecylsulfate (SDS) and urea were tested, followed by direct tryptic digestion in solution (SDC extracted proteins) and a tryptic digestion utilizing the FASP approach with molecular cut-off filters (SDS and urea extracted proteins). By this approach the SDC sample preparation protocol was classified as the most suited one to prepare truffle samples. It resulted in a high protein identification rate, had a high reproducibility, was cost efficient and most easy to handle. By a quantitative proteomic approach using label-free and non-targeted bottom up LC-MS/MS the species T. magnatum (n=13), T. aestivum (n=16), T. uncinatum (n=4) and T. melanosporum (n=7) were compared and truffle species specific markers were identified.
HostingRepository	PRIDE
AnnounceDate	2021-12-19
AnnouncementXML	Submission_2021-12-18_23:14:46.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dennis Krösser
SpeciesList	scientific name: Tuber melanosporum Mel28; NCBI TaxID: 656061;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-05 04:35:45	ID requested
⏵ 1	2021-12-18 23:14:46	announced

Dataset with its publication pending

submitter keyword: truffle, truffle species, proteomics, LC-MS/MS, food profiling

Hartmut Schlüter
contact affiliation	Universiy Medical Center Hamburg-Eppendorf, Institute for Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics
contact email	hschluet@uke.de
lab head
Dennis Krösser
contact affiliation	University Medical Center Hamburg-Eppendorf
contact email	d.kroesser@uke.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017901
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics of different truffle species