PXD017895

PXD017895 is an original dataset announced via ProteomeXchange.

Title	Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry
Description	Tissue morphogenesis requires the spatial control over actomyosin contractility to drive cell shape changes. How developmental patterning information controls cell mechanics is poorly understood. In the Drosophila embryo ectoderm, Myosin-II is enriched at the interface between antero-posterior neighboring cells, leading to planar polarized cell intercalation. G protein-coupled receptors (GPCRs) are required for planar polarized Myosin-II activation at junctions and Toll receptors provide a positional code underlying this process. How Toll receptors polarize actomyosin contractility remains unknown. Here we report that cells expressing different levels of a single Toll receptor Toll-8 activate Myosin-II at their interface. Surprisingly, the Toll-8 intracellular domain is not required for signaling at cell interfaces suggesting signaling by proxy. We found that Toll-8 forms a molecular complex with the adhesion GPCR Cirl/Latrophilin that is required for Toll-8 dependent junctional Myosin-II activation. Strikingly, the interfaces between Cirl expressing and cirl mutant cells also activate Myosin-II suggesting that Toll-8 induces Cirl asymmetric signaling at cell interfaces. We further showed that Toll-8 recruits Cirl both in trans and in cis, inducing asymmetric Cirl localization at the boundary of the Toll-8 expression domain. Finally, we found that Toll-8 and Cirl exhibit dynamic interdependent planar polarization when neighboring cells express different levels of Toll-8. Through this feedback, Toll-8 and Cirl self-organize planar polarized signaling.
HostingRepository	PRIDE
AnnounceDate	2021-04-23
AnnouncementXML	Submission_2021-05-27_21:58:06.885.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-05 03:28:15	ID requested
1	2021-04-22 10:44:24	announced
2	2021-04-22 21:45:34	announced	2021-04-23: Updated project metadata.
⏵ 3	2021-05-27 21:58:07	announced	2021-04-23: Updated project metadata. 2021-05-28: Updated publication reference for PubMed record(s): 33932333.

Lavalou J, Mao Q, Harmansa S, Kerridge S, Lellouch AC, Philippe JM, Audebert S, Camoin L, Lecuit T, Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry. Dev Cell, 56(11):1574-1588.e7(2021) [pubmed]

submitter keyword: LC-MSMS, Cirl, Myo-II, LRR,Drosophila, planar polarity, Toll-8, GPCR

Audebert Stephane
contact affiliation	Marseille Proteomic, CRCM, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105,Institut Paoli Calmettes 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	stephane.audebert@inserm.fr
lab head
AUDEBERT Stephane
contact affiliation	Pharmacology department
contact email	stephane.audebert@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD017895

PRIDE project URI

• PRIDE
  1. PXD017895
     1. Label: PRIDE project
     2. Name: Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry