PXD017886

PXD017886 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics on spermathecal fluid of BC queens
Description	We sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the spermathecal fluid (sperm removed). Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buffer. The sample was then diluted 4x with ammonium bicarbonated and digested overnight with trypsin. 2 ug of peptides were analyzed by an EASY-nLC 1000 liquid chromatography system coupled to a Bruker Impact II mass spectrometer. Data were searched with MaxQuant and the zipped txt folder output is provided.
HostingRepository	MassIVE
AnnounceDate	2020-11-01
AnnouncementXML	Submission_2020-11-01_18:40:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alison McAfee
SpeciesList	scientific name: Apis mellifera;
ModificationList	unknown modification; unknown modification; unknown modification
Instrument	instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 19:39:36	ID requested
⏵ 1	2020-11-01 18:40:57	announced

Publication List

McAfee A, Milone J, Chapman A, Foster LJ, Pettis JS, Tarpy DR, Candidate stress biomarkers for queen failure diagnostics. BMC Genomics, 21(1):571(2020) [pubmed]

McAfee A, Milone J, Chapman A, Foster LJ, Pettis JS, Tarpy DR, Candidate stress biomarkers for queen failure diagnostics. BMC Genomics, 21(1):571(2020) [pubmed]

Keyword List

submitter keyword: Honey bee, Queen, Spermatheca

submitter keyword: Honey bee, Queen, Spermatheca

Contact List

Leonard Foster
contact affiliation	University of British Columbia
contact email	foster@msl.ubc.ca
lab head
Alison McAfee
contact affiliation	University of British Columbia
contact email	alison.mcafee@alumni.ubc.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085056/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085056/