PXD017884

PXD017884 is an original dataset announced via ProteomeXchange.

Title	MitoPlex: A Targeted Multiple Reaction Monitoring Assay for Quantification of a Curated Set of Mitochondrial Proteins
Description	Mitochondria are the major source of cellular energy (ATP), as well as critical mediators of widespread functions such as cellular redox balance, apoptosis, and metabolic flux. Methods to quantify mitochondrial content are limited to low throughput immunoassays, measurement of mitochondrial DNA, or relative quantification by untargeted mass spectrometry. Here, we present a high throughput, focused multiple reaction monitoring based assay of 32 proteins critical to central carbon chain metabolism and overall mitochondrial function termed ‘MitoPlex’. We coupled this protein multiplex with a parallel analysis of 218 metabolites extracted in tandem from the same sample. In tests of its biological applicability, “MitoPlex plus metabolites” indicated profound effects of HMG-CoA Reductase inhibition (e.g., statin treatment) on mitochondria of differentiating C2C12 skeletal myoblasts, as well as a clear opposite trend of statins to promote mitochondrial protein expression and metabolism in heart and liver, while suppressing mitochondrial protein and aspects of metabolism in the skeletal muscle of C57Bl6 mice. Our results not only reveal new insights into the metabolic effect of statins in skeletal muscle, but present a new high throughput, reliable MS-based tool to study mitochondrial dynamics in both cell culture and in vivo models.
HostingRepository	PanoramaPublic
AnnounceDate	2020-04-09
AnnouncementXML	Submission_2020-04-09_06:11:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Sarah Parker
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	Phospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(5)15N(1); Label:13C(6)15N(1)
Instrument	QTRAP 6500+; TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 15:25:30	ID requested
⏵ 1	2020-04-09 06:11:02	announced

Stotland AB, Spivia W, Orosco A, Andres AM, Gottlieb RA, Van Eyk JE, Parker SJ, MitoPlex: A targeted multiple reaction monitoring assay for quantification of a curated set of mitochondrial proteins. J Mol Cell Cardiol, 142():1-13(2020) [pubmed]

submitter keyword: Mitoplex, MRM, metabolomics, statin myopathy, striated muscle, mitochondrial function

Sarah Parker
contact affiliation	Cedars-Sinai Medical Center
contact email	sarah.parker@cshs.org
lab head
Sarah Parker
contact affiliation	Cedars-Sinai Medical Center
contact email	sarah.parker@cshs.org
dataset submitter

Panorama Public dataset URI