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PXD017881

PXD017881 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEstablishment of a novel in vitro transcription assay
DescriptionRNA Polymerase II (Pol II) transcriptional recycling is an underappreciated mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptional recycling factors and quantitative measurement of transcriptional output from recycled transcriptional components. By combing our in vitro transcription assays with other experiments (e.g. in vivo dynamic ChIP-seq assays), we identified PAF1 complex components and phospho-MED1 as drivers for transcription recycling, revealing a new layer in controlling Pol II-dependent transcription. Our findings also point to RNA Pol II transcription recycling as a mechanism that functions aberrantly in disease states such as cancer, and indicate the potential to target factors such as PAF1 and phospho-MED1 that drive RNA Pol II recycling in cancer.
HostingRepositoryPRIDE
AnnounceDate2021-11-03
AnnouncementXMLSubmission_2021-11-03_05:16:03.779.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD017881
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterZhong Chen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListDeamidated; Oxidation; monohydroxylated residue; deaminated residue; Carbamidomethyl; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-04 03:34:56ID requested
12021-11-03 05:16:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: In vitro transcription, LC-MS/MS
Contact List
Qianben Wang
contact affiliationDepartment of Pathology and Duke Cancer Institute, Duke University School of Medicine, Durham, NC 27710, USA
contact emailqianben.wang@duke.edu
lab head
Zhong Chen
contact affiliationDuke University
contact emailzhong.chen128@duke.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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