PXD017878

PXD017878 is an original dataset announced via ProteomeXchange.

Title	Modern and Fossil Coral Skeletal Proteomes - Fossil Soluble Trypsin2
Description	Here we report the first recovery, sequencing, and identification of fossil biomineral proteins from a Pleistocene invertebrate. Fossils of the Caribbean stony coral Orbicella annularis retain total hydrolyzable amino acids of a similar composition to extracts from modern O. annularis skeletons and ~10% of the modern skeletal proteome was sequenced by LC-MS/MS over multiple trials in the best-preserved fossil coral specimen. The data are rich in acidic amino acids such as aspartate and glutamate typical of skeletal proteins, and one of the four sequenced fossil proteins, a highly acidic protein, has been previously characterized in modern coral skeletons. A combination of degradation, or amino acid racemization inhibition of trypsin digestion, appears to limit greater recovery. Nevertheless, our workflow determines optimal samples for effective sequencing of fossil coral proteins, allowing comparison of modern and fossil invertebrate protein sequences, and will likely lead to further improvements of the methods. Sequencing of endogenous organic molecules in fossil biominerals provides an ancient record of composition, potentially clarifying evolutionary changes and biotic responses to paleoenvironments.
HostingRepository	PRIDE
AnnounceDate	2020-10-27
AnnouncementXML	Submission_2020-10-26_23:54:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeana Drake
SpeciesList	scientific name: Orbicella annularis; NCBI TaxID: 48500;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 03:14:57	ID requested
⏵ 1	2020-10-26 23:54:06	announced

Dataset with its publication pending

submitter keyword: Scleractinia, Cnidaria, Pleistocene, highly acidic proteins, Orbicella annularis

David Jacobs
contact affiliation	Department of Ecology & Evolutionary Biology, University of California, Los Angeles, CA
contact email	djacobs@g.ucla.edu
lab head
Jeana Drake
contact affiliation	University of California, Los Angeles University of Haifa
contact email	jeanadrake@g.ucla.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD017878

PRIDE project URI

• PRIDE
  1. PXD017878
     1. Label: PRIDE project
     2. Name: Modern and Fossil Coral Skeletal Proteomes - Fossil Soluble Trypsin2