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PXD017839

PXD017839 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and design of immunotherapeutics
DescriptionThe features of peptide antigens that contribute to their immunogenicity are poorly understood. Although the stability of peptide-MHC (pMHC) is known to be important, current assays assess this interaction only for peptides in isolation and not in the context of natural antigen processing and presentation. Here, we present a novel method which provides a comprehensive and unbiased measure of pMHC stability for thousands of individual ligands detected simultaneously by mass spectrometry (MS). The method allows rapid assessment of intra- and inter-allelic differences in pMHC stability and reveals broader profiles of stability than previously appreciated. The additional dimensionality of the data facilitated the training of a model which improved the prediction of peptide immunogenicity, specifically of cancer neoepitopes. This assay can be applied to any cells bearing MHC or MHC-like molecules, offering insight into not only the endogenous immunopeptidome, but also that of neoepitopes and pathogen-derived sequences.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:15:51.960.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmma Jappe
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-03-03 01:42:27ID requested
12020-11-30 00:20:12announced
22020-12-15 22:26:27announced2020-12-16: Updated publication reference for PubMed record(s): 33298915.
32024-10-22 05:15:54announced2024-10-22: Updated project metadata.
Publication List
Jappe EC, Garde C, Ramarathinam SH, Passantino E, Illing PT, Mifsud NA, Trolle T, Kringelum JV, Croft NP, Purcell AW, Thermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and aid for immunotherapy design. Nat Commun, 11(1):6305(2020) [pubmed]
10.1093/nar/gky1106;
Keyword List
submitter keyword: MHC, thermostability, immunopeptidome
Contact List
Anthony Wayne Purcell
contact affiliationBiomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
contact emailanthony.purcell@monash.edu
lab head
Emma Jappe
contact affiliationEvaxion Biotech, Technical University of Denmark
contact emailemja@evaxion-biotech.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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