PXD017837

PXD017837 is an original dataset announced via ProteomeXchange.

Title	Characterization of Bovine Dry Secretions and their Proteome from the End of Lactation Through Day 21 of the Dry Period
Description	The dry period in cow are critical to mammary health and the success of the next lactation. The expression of proteins the dry period progresses need to be characterized to provide information on involution and immune functions during the dry period. Total protein and lactoferrin concentrations rose significantly by days 10 and 21 after cessation of lactation. Using the day 21 dry secretions we examined their ability to inhibit bacterial growth. Bacterial growth inhibition was not correlated with lactoferrin for 4 coliforms tested nor for all 7 mastitis pathogens. Label free mass spectroscopy was used to quantify changes in whey proteins from milk/dry secretions collected on days 0, 3,10, and 21 of the dry period. 776 proteins were identified. 109 proteins were upregulated at one or more times in the dry period. The most significate enriched GO functional terms for the up-regulated proteins were immune function and stress related. 68 proteins were down regulated in the dry period. The most significant enriched GO functional terms for the down-regulated proteins were stress and immune function related. 12 proteins were significantly correlated with coliform growth. The dry secretion composition changes we found provide information for understanding the dry period. The sample code for the MS raw files is as follows. The 3 technical replicates are denoted as a letter A, B and C. The number following is the cow identification number for 11 cows used. The final two-digit number after the underscore is the day sampled where _01 = day 1, _03 = day 3, _10 = day 10 and _21 = day 21 of dry period. For example, A1313_01 is technical replicate A for cow 1313 collected on day 1. B1313_03 is technical replicate B for cow 1313 collected on day 3.
HostingRepository	PRIDE
AnnounceDate	2020-07-22
AnnouncementXML	Submission_2020-08-11_05:31:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Lippolis
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 23:43:19	ID requested
1	2020-05-20 04:32:52	announced
2	2020-07-22 04:43:44	announced	2020-07-22: Updated project metadata.
⏵ 3	2020-08-11 05:31:39	announced	2020-07-22: Updated project metadata. 2020-08-11: Updated publication reference for PubMed record(s): 32671157.

Reinhardt TA, Lippolis JD, Dataset of bovine mammary gland dry secretion proteome from the end of lactation through day 21 of the dry period. Data Brief, 31():105954(2020) [pubmed]

submitter keyword: Dairy cow dry period, bovine dry secretions, proteome, mastitis, mammary involution

Timothy A. Reinhardt
contact affiliation	Ruminant Diseases and Immunology Unit, National Animal Disease Center, USDA/ARS, Ames IA, 50010 United States
contact email	tim.reinhardt@usda.gov
lab head
John Lippolis
contact affiliation	USDA/ARS/NADC
contact email	john.lippolis@ars.usda.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017837

PRIDE project URI

• PRIDE
  1. PXD017837
     1. Label: PRIDE project
     2. Name: Characterization of Bovine Dry Secretions and their Proteome from the End of Lactation Through Day 21 of the Dry Period