PXD017828

PXD017828 is an original dataset announced via ProteomeXchange.

Title	Proteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum
Description	The archaea Cuniculiplasma divulgatum are abundant in acidic environments with low to moderate temperatures. However, the molecular mechanisms underlying its ability to thrive at low temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold shock. The C. divulgatum genome encodes 2,016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of C. divulgatum, its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock (the core proteome), whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in protein levels was found for the metal-dependent hydrolase, FAD-dependent oxidoreductase, aspartate carbamoyltransferase regulatory chain proteins, 2-oxoacid ferredoxin oxidoreductase and ATPase-V type ATP synthase. Furthermore, the cold shock induced a substantial increase (3.5 % and 9.0 %) in the levels of two most abundant intracellular proteins, chaperonin and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of Cuniculiplasma spp. allowing them to colonise and survive in acidic settings at low/ moderate temperatures.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_06:45:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017828
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rafael Bargiela
SpeciesList	scientific name: Cuniculiplasma divulgatum; NCBI TaxID: 1673428;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; deaminated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 06:08:42	ID requested
⏵ 1	2020-05-26 06:45:04	announced

Bargiela R, Lanthaler K, Potter CM, Ferrer M, Yakunin AF, Paizs B, Golyshin PN, Golyshina OV, . Microorganisms, 8(5):(2020) [pubmed]

submitter keyword: Acidophilic archaea, acidic environments, acid mine drainage systems, Thermoplasmatales, Cuniculiplasma, proteome, cold shock

Peter Golyshin
contact affiliation	Centre for Environmental Biotechnology, Bangor University, Deiniol Rd, Bangor, LL57 2UW, UK School of Natural Sciences, Bangor University, Deiniol Rd, Bangor, LL57 2UW, UK
contact email	p.golyshin@bangor.ac.uk
lab head
Rafael Bargiela
contact affiliation	Center for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor
contact email	f.bargiela@bangor.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017828

PRIDE project URI

• PRIDE
  1. PXD017828
     1. Label: PRIDE project
     2. Name: Proteome cold-shock response in the extremely acidophilic archaeon, Cuniculiplasma divulgatum