PXD017825

PXD017825 is an original dataset announced via ProteomeXchange.

Title	Peptidomics of sorted enteroendocrine cells from human organoids
Description	A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS
HostingRepository	PRIDE
AnnounceDate	2020-07-01
AnnouncementXML	Submission_2020-06-30_23:47:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017825
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Richard Kay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; Amidated; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 03:43:41	ID requested
⏵ 1	2020-06-30 23:47:32	announced

Dataset with its publication pending

submitter keyword: Peptidomics, glucagon, organoids

Fiona Gribble
contact affiliation	Institute of Metabolic Research, MRC Metabolic Research Laboratories, Cambridge University
contact email	fmg23@cam.ac.uk
lab head
Richard Kay
contact affiliation	University of Cambridge
contact email	rgk27@medschl.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017825

PRIDE project URI

• PRIDE
  1. PXD017825
     1. Label: PRIDE project
     2. Name: Peptidomics of sorted enteroendocrine cells from human organoids