PXD017822

PXD017822 is an original dataset announced via ProteomeXchange.

Title	The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation
Description	Alpha-herpesviruses establish a life-long infection in the nervous system of the affected host; while this infection is restricted to peripheral neurons in a heathy host, it can spread within the neuronal circuitry in compromised individuals leading to adverse health consequences. Pseudorabies virus (PRV) an alpha-herpesvirus, requires the viral protein Us9 for sorting virus particles into axons. It does so by mediating an interaction of virus particles with neuronal transport machinery. Us9-mediated axonal sorting also depends on the state of neuronal maturation as the proteome composition changes with neuronal development of dendrites and axons. Immature superior cervical ganglia (SCGs) have rudimentary neurites that lack markers of mature dendrites or axons. Immature SCGs can be infected by PRV, but show markedly reduced Us9-dependent sorting into neurites. Mature SCGs abundantly express a variety of proteins characteristic of vesicle-transport machinery. Proteomics studies identified several novel Us9-associated neuronal proteins with potential roles in the regulation of axonal sorting and subsequent anterograde spread of virus particles in axons. For example, we found that SMPD4/n-sMase3, a sphingomyelinase abundant in lipid-rafts, associates with Us9 and is a negative regulator of axonal mediated spread of PRV, a potential novel antiviral function.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_04:44:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Federspiel
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 02:48:06	ID requested
⏵ 1	2021-01-04 04:44:32	announced

Tanneti NS, Federspiel JD, Cristea IM, Enquist LW, The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation. PLoS Pathog, 16(12):e1008861(2020) [pubmed]

submitter keyword: Us9, PRV, Virus-host, DDA, IP-MS, LFQ, TMT

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Joel Federspiel
contact affiliation	Princeton University
contact email	joel.federspiel@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017822
     1. Label: PRIDE project
     2. Name: The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation The axonal sorting activity of pseudorabies virus Us9 protein depends on the state of neuronal maturation