PXD017809

PXD017809 is an original dataset announced via ProteomeXchange.

Title	Non-metabolic PHGDH facilitates cMyc to urge advanced liver cancer
Description	Metabolic dysregulation is an important feature of malignant tumors. Serine synthesis pathway (SSP) reshapes tumor cells to enhance their growth and survival capabilities, especially under serine starvation. However, whether and how SSP is involved in the progression of liver cancer has not been clearly stated. We applied a combination of quantitative proteomics and transcriptomics and discovered the increased expression of PHGDH and PSAT1 in mice liver cancer induced by SB. PHGDH is the first rating-limited enzyme of SSP and the conditional knockout of PHGDH in liver impaired the advanced liver cancer formation. However, the specific inhibition of enzymatic activity of PHGDH in liver has no obvious suppressive effect on advanced liver cancer. Further, we explored a non-metabolic function of PHGDH, which used its ACT domain to bind and regulate cMyc transcriptional activity, then selectively activated genes expression of Lcn2, Got1, Fabp5 and Psat1, indicating PHGDH drives/links multiple metabolic pathways and augmented its function through its non-metabolic role. In addition, the mutant p53 would release the suppression of PHGDH in mice liver cancer model and clinical liver cancer samples. Hence, the mutant p53/PHGDH/cMyc axis could be a potential target to cure advanced liver cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:54:50.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhu sam
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 01:53:23	ID requested
1	2023-07-20 10:49:00	announced
2	2023-11-14 09:02:35	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:54:54	announced	2024-10-22: Updated project metadata.

Zhu H, Yu H, Zhou H, Zhu W, Wang X, Elevated Nuclear PHGDH Synergistically Functions with cMyc to Reshape the Immune Microenvironment of Liver Cancer. Adv Sci (Weinh), 10(17):e2205818(2023) [pubmed]

10.1002/advs.202205818;

submitter keyword: serine synthesis, hepatocellular carcinoma,PHGDH, proteome

Xiongjun Wang
contact affiliation	Shanghai Institue of Nutrition and Health, CAS
contact email	xjwang02@sibcb.ac.cn
lab head
zhu sam
contact affiliation	sibs
contact email	zhw@simm.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD017809

PRIDE project URI

• PRIDE
  1. PXD017809
     1. Label: PRIDE project
     2. Name: Non-metabolic PHGDH facilitates cMyc to urge advanced liver cancer