PXD017806

PXD017806 is an original dataset announced via ProteomeXchange.

Title	Mapping RNA interactions of human ATRX with RepA fragment of Xist
Description	Heterochromatin in the eukaryotic genome is rigorously controlled by the concerted action of protein factors and RNAs. Here, we investigate the RNA binding function of ATRX, a chromatin remodeler with roles in silencing of repetitive regions of the genome and in recruitment of the polycomb repressive complex 2 (PRC2). We identify ATRX RNA binding regions (RBRs) and discover that the major ATRX RBR lies within the N-terminal region of the protein, distinct from its PHD and helicase domains. Deletion of this ATRX RBR (ATRXRBR) compromises ATRX interactions with RNAs in vitro and in vivo and alters its chromatin binding properties. Genome-wide studies reveal that loss of RNA interactions results in a redistribution of ATRX on chromatin. Finally, our studies identify a role for ATRX-RNA interactions in regulating PRC2 localization to a subset of polycomb target genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:56.345.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017806
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Warneford-Thomson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-02 01:23:58	ID requested
1	2020-03-09 01:00:09	announced
2	2020-05-21 23:29:07	announced	2020-05-22: Updated publication reference for PubMed record(s): 32376827.
⏵ 3	2024-10-22 04:59:58	announced	2024-10-22: Updated project metadata.

10.6019/PXD017806;

Ren W, Medeiros N, Warneford-Thomson R, Wulfridge P, Yan Q, Bian J, Sidoli S, Garcia BA, Skordalakes E, Joyce E, Bonasio R, Sarma K, Disruption of ATRX-RNA interactions uncovers roles in ATRX localization and PRC2 function. Nat Commun, 11(1):2219(2020) [pubmed]

10.1038/s41467-020-15902-9;

submitter keyword: RBRID

RNA-protein interactions

RNA-binding

Roberto Bonasio
contact affiliation	Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania
contact email	roberto@bonasiolab.org
lab head
Robert Warneford-Thomson
contact affiliation	Octant Bio
contact email	warnefordthomsonr@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD017806

PRIDE project URI

• PRIDE
  1. PXD017806
     1. Label: PRIDE project
     2. Name: Mapping RNA interactions of human ATRX with RepA fragment of Xist