PXD017799

PXD017799 is an original dataset announced via ProteomeXchange.

Title	Human urine proteome in overactive bladder syndorme
Description	The aim of this study is analysis of urinary proteomic pattern in healthy people and patients suffering from OAB symptoms by means of mass spectrophotometry. Establishing characteristic protein profiles, qualitative and quantitative, for OAB will help to define specific protein marker and will allow to develop new investigative protocol enabling to discover at what stage of post-genetic changes urothelium cells change their proteomic profile causing OAB symptoms. Patients will be divided into two groups: group A – OAB patients and group B – control group, without any urinary tract symptoms. All participants will be questionnaired with appropriate tools evaluating quality of life of patients with lower urinary tract symptoms and bladder diary. Urine samples will be taken twice with 7 day gap and then prepared for further evaluation with mass spectrophotometry. Mass spectrophotometry will be performed for all labeled samples with QExactive spectrophotometer. In order to provide maximal credibility and reliability of acquired data each sample will be managed in triplicate. Those result will be analyzed with specific statistical methods and final results will be correlated between the groups. In long-term perspective results of this project will help to understand the underlying pathomechanisms of overactive blabber syndrome. In future those results will be applied for further research in OAB sufferers allowing for precise and objective diagnosis and prognosis. In our opinion it will also serve as a tool for estimations of treatment efficacy of new therapeutic methods. In consequence the project will allow us to create a unique toolkit, combining spectrophotometry, urinary proteomic pattern differences and quality of life correlated with OAB symptoms severity. It will enable to develop new perspectives for overactive bladder pathophysiology research and new therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2020-05-12
AnnouncementXML	Submission_2020-07-22_06:50:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017799
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Konrad Futyma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; iTRAQ8plex; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-01 22:48:16	ID requested
1	2020-05-11 23:07:48	announced
⏵ 2	2020-07-22 06:50:31	announced	2020-07-22: Updated publication reference for PubMed record(s): 32397227.

Futyma K, Nowakowski Ł, Zi, ę, tek-Strobl A, Kami, ń, ska A, Taoussi N, Rechberger T, Urine Proteomic Study in OAB Patients-Preliminary Report. J Clin Med, 9(5):(2020) [pubmed]

submitter keyword: overactive bladder, Urgency urinary incontinence

Lower urinary tract symptoms

Michał Dadlez
contact affiliation	Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.
contact email	michald@ibb.waw.pl
lab head
Konrad Futyma
contact affiliation	2nd Department of Gynecology, Medical University of Lublin, Poland
contact email	futymakonrad@mp.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017799

PRIDE project URI

• PRIDE
  1. PXD017799
     1. Label: PRIDE project
     2. Name: Human urine proteome in overactive bladder syndorme