PXD017780

PXD017780 is an original dataset announced via ProteomeXchange.

Title	Common and strain-specific post-translational modifications of the potyvirus Plum pox virus coat protein in different hosts
Description	Phosphorylation and O-GlcNAcylation are widespread post-translational modifications (PTMs) often sharing protein targets. Numerous studies have reported phosphorylation of plant virus proteins. In plants, research on O-GlcNAcylation lags behind regarding other eukaryotes and information about O-GlcNAcylated plant viral proteins is extremely scarce. The potyvirus Plum pox virus (PPV) causes sharka disease in Prunus trees, and also infects a wide range of experimental hosts. Capsid protein (CP) from virions of the PPV-R isolate purified from herbaceous plants can be extensively modified by O-GlcNAcylation and phosphorylation. In this study, a combination of proteomics and biochemical approaches has been employed to broaden knowledge of PPV CP PTMs. CP proved to be modified regardless it is assembled or not in mature particles. PTMs of CP occur in the natural host Prunus persica, similar to what happens in herbaceous plants. Additionally, we observe O-GlcNAcylation and phosphorylation are general features of different PPV strains, suggesting that roles of these modifications are part of overall strategies deployed during plant-virus interactions. Interestingly, phosphorylation at a casein kinase II motif conserved among potyviral CPs exhibits strain specificity in PPV; however, it does not display the critical role attributed to same modification in the CP of another potyvirus, Potato virus A.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_15:59:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017780
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Plum pox virus; NCBI TaxID: 12211;
ModificationList	Phospho; Methylthio; Deamidated; phosphorylated residue; Oxidation; HexNAc; Carbamyl; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-28 04:38:58	ID requested
⏵ 1	2020-05-26 15:59:53	announced

Herv, á, s M, Ciordia S, Navajas R, Garc, í, a JA, Mart, í, nez-Turi, ñ, o S, Coat Protein in Different Hosts. Viruses, 12(3):(2020) [pubmed]

submitter keyword: Capsid protein

O-GlcNAcylation

phosphorylation

sharka

potyvirus

Juan Antonio García
contact affiliation	Department of Plant Molecular Genetics, Centro Nacional de Biotecnología (CNB-CSIC),Madrid, Spain.
contact email	jagarcia@cnb.csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017780
     1. Label: PRIDE project
     2. Name: Common and strain-specific post-translational modifications of the potyvirus Plum pox virus coat protein in different hosts