PXD017776

PXD017776 is an original dataset announced via ProteomeXchange.

Title	Tuning Liposome Composition to Modulate the Corona Forming in Human Serum and Uptake by Cells
Description	Nano-sized objects such as liposomes are modified by adsorption of biomolecules in biological fluids. The resulting corona critically changes nanoparticle behavior at cellular level. A better control of corona composition could allow to modulate uptake by cells. Within this context, in this work, liposomes of different charge were prepared by mixing negatively charged and zwitterionic lipids to different ratios. The series obtained was used as a model system with tailored surface properties to modulate corona composition and determine the effects on liposome interactions with cells. Uptake efficiency and uptake kinetics of the different liposomes were determined by flow cytometry and fluorescence imaging. Particular care was taken in optimizing the methods to isolate the corona forming in human serum to prevent liposome agglomeration and to exclude residual free proteins which could confuse the results. Thanks to the optimized methods, mass spectrometry of replicate corona isolations showed excellent reproducibility and this allowed semi-quantitative analysis to determine for each formulation the most abundant proteins in the corona. The results showed that by changing the fraction of zwitterionic and charged lipids in the bilayer, the amount and identity of the most abundant proteins adsorbed from serum differed. Interestingly, the formulations also showed very different uptake kinetics. Similar approaches can be used to tune lipid composition in a systematic way in order to obtain formulations with the desired corona and cell uptake behavior.
HostingRepository	PRIDE
AnnounceDate	2020-02-28
AnnouncementXML	Submission_2020-02-28_05:02:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017776
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Horvatovich Péter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-28 03:21:56	ID requested
⏵ 1	2020-02-28 05:02:24	announced

Yang K, Mesquita B, Horvatovich P, Salvati A, Tuning liposome composition to modulate corona formation in human serum and cellular uptake. Acta Biomater, 106():314-327(2020) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Liposomes, nanoparticle corona, human serum, cellular uptake

Anna Salvati, Peter Horvatovich
contact affiliation	University of Groningen. GRIP.
contact email	p.l.horvatovich@rug.nl
lab head
Horvatovich Péter
contact affiliation	Faculty of Mathematics and Natural Sciences, Department of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands
contact email	p.l.horvatovich@rug.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017776
     1. Label: PRIDE project
     2. Name: Tuning Liposome Composition to Modulate the Corona Forming in Human Serum and Uptake by Cells