PXD017775

PXD017775 is an original dataset announced via ProteomeXchange.

Title	proteome analysis using mass spectrometry (MS) in acute shYthdf2-mediated knockdown of cultured RGCs
Description	To identify mouse retinal proteomics changes after Ythdf2 knockdown, we extracted the proteomics of E15.5 retina neurons which were cultured and infected with lentiviral shYthdf2 or shCtrl by urea. After using 100 mM TEAB (Sigma) to dilute the urea concentration to less than 2 M in each sample, trypsin (Promega) was then added to digest the proteins overnight at 37 °C. Peptides were further desalted by Strata X C18 SPE column (Phenomenex) and labelled with TMT10plex Mass Tag Labelling kit (Thermo Scientific) according to the manufactural instructions. Finally, the labeled peptides were subjected to HPLC fractionation and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_04:17:50.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fugui Niu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-28 03:21:48	ID requested
⏵ 1	2022-05-25 04:17:51	announced

Niu F, Han P, Zhang J, She Y, Yang L, Yu J, Zhuang M, Tang K, Shi Y, Yang B, Liu C, Peng B, Ji SJ, A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. Elife, 11():(2022) [pubmed]

submitter keyword: Mouse

Retina

Ythdf2

Knockdown

Shengjian Ji
contact affiliation	Southern University of Science and Technology
contact email	11849496@mail.sustech.edu.cn
lab head
Fugui Niu
contact affiliation	Southern Unversity of Science and Technology
contact email	11849496@mail.sustech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD017775

PRIDE project URI

• PRIDE
  1. PXD017775
     1. Label: PRIDE project
     2. Name: proteome analysis using mass spectrometry (MS) in acute shYthdf2-mediated knockdown of cultured RGCs