PXD017768

PXD017768 is an original dataset announced via ProteomeXchange.

Title	Impact of subclinical intramammary infection by coagulase-negative staphylococci on the bovine milk peptidome
Description	Previous studies have investigated the peptidomic changes occurring in cow milk during mastitis; however, these focused mainly on clinical mastitis, either spontaneous (Mansor et al., 2013) or induced by experimental infection (Thomas et al., 2016). Mansor and coworkers were the first to use mass spectrometry to demonstrate that several peptides found increased in milk from cows with clinical S. aureus or E. coli mastitis were mainly derived from aS1- and b-casein. In that study, 48 peptides were significantly different between the milks of healthy and mastitic cows. Non-mastitic samples were confirmed to be non-mastitic by having SCC <100,000 cells/mL (Mansor et al., 2013). Thomas and coworkers expanded the peptidomic repertoire in a study evaluating the kinetics of experimental S. uberis infection, and found signature peptides with potential as mastitis markers (Thomas et al., 2016). Only one study evaluated the milk peptidome in subclinical mastitis (Guerrero et al., 2015) demonstrating that even subclinical infections can cause significant increases in the total number of released peptides when compared to uninfected milk. However, neither the IMI agents nor the somatic cell counts were reported. With the aim of understanding high abundance protein and peptidomic changes due to subclinical CNS mastitis, to identify signature peptides with potential for subclinical mastitis detection, and to compare the proteomic and peptidomic findings with those reported in clinical mastitis, we investigated the influence of CNS IMI on high abundance milk proteins by SDS-PAGE and densitometric analysis, followed by a detailed characterization of the milk peptidome by means of high-performance liquid chromatography/tandem mass spectrometry and bioinformatic analysis.
HostingRepository	PRIDE
AnnounceDate	2020-07-16
AnnouncementXML	Submission_2020-07-16_03:51:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elisa Maffioli
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-27 07:52:41	ID requested
⏵ 1	2020-07-16 03:51:04	announced

Addis MF, Maffioli EM, Ceciliani F, Tedeschi G, Zamarian V, Tangorra F, Albertini M, Piccinini R, Bronzo V, Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome. J Proteomics, 226():103885(2020) [pubmed]

submitter keyword: peptidomics;Subclinical mastitis

coagulase-negative staphylococci

LC-MS/MS

elisa maffioli
contact affiliation	Department of Veterinary Medicine, University of Milan
contact email	elisa.maffioli@unimi.it
lab head
Elisa Maffioli
contact affiliation	Università degli Studi di Milano
contact email	elisa.maffioli@unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017768

PRIDE project URI

• PRIDE
  1. PXD017768
     1. Label: PRIDE project
     2. Name: Impact of subclinical intramammary infection by coagulase-negative staphylococci on the bovine milk peptidome