PXD017762

PXD017762 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The proteome of Rickettsia akari revealed a 44kDa-OMP as novel biomarker for Rickettsialpox
Description	Our study aimed to investigate the proteome structure of R. akari and unveil immunoreactive surface-exposed proteins using the latest proteomics techniques. To achieve these goals, we applied the Shotgun protein identification approach; membrane surface protein enrichment methods combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoblotting techniques. We identified aroun 300 unique proteins from the whole-cell extract exhibiting that the most represented proteins were those involved in translation, post-translational modifications, energy production, and cell wall development. A significant part belongs to amino acid transport and intracellular trafficking. Besides, several proteins affecting virulence were detected. Then, in silico analysis from detected surface proteins revealed that 25 putative predicted outer membrane proteins contain beta-barrel structure and 11 proteins were predicted to have secretion signal peptide sequence.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:09:06.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiri DRESLER
SpeciesList	scientific name: Rickettsia akari; NCBI TaxID: 786;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-27 03:14:46	ID requested
1	2020-07-16 02:33:12	announced
⏵ 2	2024-10-22 05:09:12	announced	2024-10-22: Updated project metadata.

Publication List

10.1186/s12866-020-01877-6;
Csicsay F, Flores-Ramirez G, Zu, ñ, iga-Navarrete F, Barto, š, ov, á M, Fu, č, í, kov, á A, Pajer P, Dresler J, Š, kult, é, ty Ľ, Quevedo-Diaz M, kDa-OMP as a potential biomarker for Rickettsialpox diagnosis. BMC Microbiol, 20(1):200(2020) [pubmed]

10.1186/s12866-020-01877-6;

Csicsay F, Flores-Ramirez G, Zu, ñ, iga-Navarrete F, Barto, š, ov, á M, Fu, č, í, kov, á A, Pajer P, Dresler J, Š, kult, é, ty Ľ, Quevedo-Diaz M, kDa-OMP as a potential biomarker for Rickettsialpox diagnosis. BMC Microbiol, 20(1):200(2020) [pubmed]

Keyword List

submitter keyword: egg culture, Q-Exactive, VERO cell line,Rickettsia akari

submitter keyword: egg culture, Q-Exactive, VERO cell line,Rickettsia akari

Contact List

Jiri Dresler
contact affiliation	Military Health Institute, Prague, Czech Republic
contact email	jiri.dresler@gmail.com
lab head
Jiri DRESLER
contact affiliation	Military Health Institute, Prague/Czech Republic
contact email	jiri.dresler@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017762
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017762

PRIDE project URI

Repository Record List

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