PXD017759

PXD017759 is an original dataset announced via ProteomeXchange.

Title	HPLC-ESI-MS PROFILE OF SALIVARY PROTEINS IN MASTOCYTOSIS PATIENTS
Description	Mastocytosis is a myeloproliferative neoplasm characterized by infiltration of clonally derived mast cells in different tissues. According to mast cells localization, it is possible to discriminate cutaneous mastocytosis (CM) from systemic mastocytosis (SM), the latter involving at least an extracutaneous organ, like bone marrow, liver, spleen and gastrointestinal tract. Some of the SM patient can develop also cutaneous lesions (SM+C). Disease classification is often tricky. Oral cavity is commonly involved in the symptomatology. With the aim to highlight possible qualitative/quantitative modifications of the salivary proteome associated to the different forms of the disease, we investigated the protein profiles of CM, SM, and SM+C patients, and healthy controls, by integrated top-down platforms. a top-down proteomic platform, based on high performance liquid chromatography separation coupled to electrospray-ion trap mass spectrometry (HPLC-ESI-IT-MS) analysis of the acidic soluble proteome of human saliva, which was standardized in our previous studies for detecting and quantifying of hundreds salivary peptides/proteins. By this approach, it is possible to obtain a profiling of the naturally occurring salivary proteome, including isoforms and post-translational modifications (PTMs), to compare an unlimited number of samples and to quantify proteins aby a label-free method.
HostingRepository	PRIDE
AnnounceDate	2020-06-25
AnnouncementXML	Submission_2020-06-24_22:48:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Serrao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); S-nitrosyl-L-cysteine; O-phospho-L-threonine; disulfide crosslinked residues; N-acetylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); O-phospho-L-serine; L-cysteine glutathione disulfide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-27 00:24:55	ID requested
⏵ 1	2020-06-24 22:48:46	announced

Serrao S, Firinu D, Olianas A, Deidda M, Contini C, Iavarone F, Sanna MT, Boroumand M, Amado F, Castagnola M, Messana I, Del Giacco S, Manconi B, Cabras T, Top-Down Proteomics of Human Saliva Discloses Significant Variations of the Protein Profile in Patients with Mastocytosis. J Proteome Res, 19(8):3238-3253(2020) [pubmed]

submitter keyword: HPLC-MSMS, salivary proteins, mastocytosis

Tiziana Cabras
contact affiliation	Department of Life and Environmental Sciences - Biomedical Section
contact email	tcabras@unica.it
lab head
Simone Serrao
contact affiliation	Life and Environmental Sciences Department (Biomedical Section) - University of Cagliari
contact email	simone.serrao@unica.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017759

PRIDE project URI

• PRIDE
  1. PXD017759
     1. Label: PRIDE project
     2. Name: HPLC-ESI-MS PROFILE OF SALIVARY PROTEINS IN MASTOCYTOSIS PATIENTS