PXD017741

PXD017741 is an original dataset announced via ProteomeXchange.

Title	A new regulatory interface on RIPK2 controls XIAP binding and NOD signaling activity
Description	Signaling via the intracellular pathogen receptors Nucleotide-binding oligomerization domain-containing proteins NOD1 and NOD2 requires Receptor Interacting Kinase 2 (RIPK2), an adaptor kinase that can be targeted for the treatment of various inflammatory diseases. However, the molecular mechanisms of how RIPK2 contributes to NOD signaling are not completely understood. We generated FLAG-tagged RIPK2 knock-in mice using CRISPR/Cas9 technology to study NOD signaling mechanisms on an endogenous level. Using cells from these mice we were able to generate a detailed map of post-translational modifications on RIPK2 during NOD signaling using mass spectrometry analysis and we identified a new regulatory interface on RIPK2, which dictates the crucial interaction with the E3 ligase XIAP.
HostingRepository	PRIDE
AnnounceDate	2020-08-13
AnnouncementXML	Submission_2020-08-12_22:29:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-26 02:57:17	ID requested
⏵ 1	2020-08-12 22:29:52	announced

Dataset with its publication pending

submitter keyword: XIAP, RIPK2, ubiquitination

Ueli Nachbur
contact affiliation	Inflammation Division The Walter and Eliza Hall Institute of Medical Researc 1G Royal Parade Parkville VIC 3052 Australia
contact email	nachbur@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017741

PRIDE project URI

• PRIDE
  1. PXD017741
     1. Label: PRIDE project
     2. Name: A new regulatory interface on RIPK2 controls XIAP binding and NOD signaling activity