PXD017738 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis demonstrated transcription factor gene YRR1 deletion in Saccharomyces cerevisiae enhances its resistance to vanillin through the upregulation of transcriptional activator Haa1, coactivator Mbf1, and proteasome assembly chaperone Tma17 expression |
Description | Vanillin is one of the major phenolic inhibitors of Saccharomyces cerevisiae in lignocellulosic hydrolysates. Deleting transcription factor gene YRR1 improves vanillin resistance by promoting some translation-related processes that were confirmed at the transcription level in our previous studies. In this work, we investigated the effect of proteomic change on vanillin stress and YRR1 deletion. In wild-type cells, vanillin reduced the number of ribosomal proteins and thereby inhibited cells’ translation. YRR1 deletion increased 112 protein quantities; 48 of 112 up-regulated proteins are involved in the stress response, translational and basal transcriptional regulation. Fermentation data showed that the overexpression of HAA1, MBF1, and TMA17, which encode transcriptional activator, coactivator, and proteasome assembly chaperone, respectively, enhanced resistance to vanillin in S. cerevisiae. These results enriched the perspective of molecular mechanisms for YRR1 deletion to protect yeast from vanillin stress and offered novel targets for designing inhibitor-resistant ethanologenic yeast strains. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:27:11.323.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xinning Wang |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-26 02:44:44 | ID requested | |
1 | 2021-09-08 17:57:40 | announced | |
⏵ 2 | 2024-10-22 05:27:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1186/s12934-021-01633-z; |
Cao W, Zhao W, Yang B, Wang X, Shen Y, Wei T, Qin W, Li Z, Bao X, Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae. Microb Cell Fact, 20(1):142(2021) [pubmed] |
Keyword List
submitter keyword: YRR1 |
quantitative proteomics |
vanillin resistance |
bioethanol |
Saccharomyces cerevisiae |
Contact List
Xinning Wang |
contact affiliation | School of Biotechnology, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China |
contact email | xnwang@qlu.edu.cn |
lab head | |
Xinning Wang |
contact affiliation | Qilu University of Technology, Shandong Academy of Sciences |
contact email | xnwang@qlu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017738
- Label: PRIDE project
- Name: Proteomic analysis demonstrated transcription factor gene YRR1 deletion in Saccharomyces cerevisiae enhances its resistance to vanillin through the upregulation of transcriptional activator Haa1, coactivator Mbf1, and proteasome assembly chaperone Tma17 expression