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PXD017736

PXD017736 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTandem mass tag-based (TMT) quantitative proteomics analyses reveal the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
DescriptionCotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. In semi-arid/arid regions, drought stress causes growth limitation and decrease of yield. Of all the organs of a plant, fine root is the central part consisting of the root system to contribute to plant water and nutrient taken up. However, the research on the molecular mechanism underlying fine root response to soil drought has not been well understood in cotton. To better characterize the proteomic changes of cotton fine roots under drought stress, 70±5% and 40±5% soil relative water content were designed as control (CK) and drought stress (DS) groups, respectively. Tandem mass tags (TMT) technology was used to determine the proteome profiles in fine roots. The proteomic differences between CK and DS were pairwise compared at 0, 30, and 45 days after drought stress (DAD). A total of 11,628 proteins were identified, of which 10,344 proteins contained quantitative information. According to the morphological, physiological, and biochemical characteristics, 30 and 45 DAD were selected as critical stages for further analysis. Results showed that 118 differentially expressed proteins (DEPs) were up-regulated and 105 down-regulated in DS 30 versus CK 30; 662 DEPs were up-regulated, and 611 were down-regulated in DS 45 versus CK 45. The DEP functions were determined for their classified pathways, mainly associated with carbohydrate metabolism, energy metabolism, fatty acid metabolism, amino acid metabolism, and secondary metabolite biosynthesis. DEPs related to phytohormone and stress/defense response were also identified. To verify the accuracy of the TMT results, 20 DEPs were randomly selected for parallel reaction monitoring (PRM) verification. And results showed that the quantitative results of TMT are consistent with those of PRM, which proved that the TMT results of this study are reliable. In this article we describe changes in the protein profiles occurring in response to drought stress in cotton fine roots. Proteomic analyses of plant responses to stressors could lead to the introduction of cotton cultivars with high resistance to drought stress. Such plants would be valuable for high yielding under drought as well as other unfavorable environmental conditions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:07:39.278.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiantao Liu
SpeciesList scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-26 02:44:31ID requested
12020-06-23 22:28:55announced
22024-10-22 05:07:39announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PRM
Proteome
Drought stress,Cotton
Fine root
TMT
Contact List
Liantao Liu
contact affiliationHebei Agricultural University
contact emailliultday@126.com
lab head
Liantao Liu
contact affiliationHebei Agricultural University
contact emailliultday@126.com
dataset submitter
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Dataset FTP location
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