PXD017736

PXD017736 is an original dataset announced via ProteomeXchange.

Title	Tandem mass tag-based (TMT) quantitative proteomics analyses reveal the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)
Description	Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. In semi-arid/arid regions, drought stress causes growth limitation and decrease of yield. Of all the organs of a plant, fine root is the central part consisting of the root system to contribute to plant water and nutrient taken up. However, the research on the molecular mechanism underlying fine root response to soil drought has not been well understood in cotton. To better characterize the proteomic changes of cotton fine roots under drought stress, 70±5% and 40±5% soil relative water content were designed as control (CK) and drought stress (DS) groups, respectively. Tandem mass tags (TMT) technology was used to determine the proteome profiles in fine roots. The proteomic differences between CK and DS were pairwise compared at 0, 30, and 45 days after drought stress (DAD). A total of 11,628 proteins were identified, of which 10,344 proteins contained quantitative information. According to the morphological, physiological, and biochemical characteristics, 30 and 45 DAD were selected as critical stages for further analysis. Results showed that 118 differentially expressed proteins (DEPs) were up-regulated and 105 down-regulated in DS 30 versus CK 30; 662 DEPs were up-regulated, and 611 were down-regulated in DS 45 versus CK 45. The DEP functions were determined for their classified pathways, mainly associated with carbohydrate metabolism, energy metabolism, fatty acid metabolism, amino acid metabolism, and secondary metabolite biosynthesis. DEPs related to phytohormone and stress/defense response were also identified. To verify the accuracy of the TMT results, 20 DEPs were randomly selected for parallel reaction monitoring (PRM) verification. And results showed that the quantitative results of TMT are consistent with those of PRM, which proved that the TMT results of this study are reliable. In this article we describe changes in the protein profiles occurring in response to drought stress in cotton fine roots. Proteomic analyses of plant responses to stressors could lead to the introduction of cotton cultivars with high resistance to drought stress. Such plants would be valuable for high yielding under drought as well as other unfavorable environmental conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:39.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liantao Liu
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-26 02:44:31	ID requested
1	2020-06-23 22:28:55	announced
⏵ 2	2024-10-22 05:07:39	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: PRM

Proteome

Drought stress,Cotton

Fine root

TMT

Liantao Liu
contact affiliation	Hebei Agricultural University
contact email	liultday@126.com
lab head
Liantao Liu
contact affiliation	Hebei Agricultural University
contact email	liultday@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017736
     1. Label: PRIDE project
     2. Name: Tandem mass tag-based (TMT) quantitative proteomics analyses reveal the response of fine roots to drought stress in cotton (Gossypium hirsutum L.)