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PXD017723

PXD017723 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFyn- and Abl-driven interactome and post-translational modifications of DCBLD1 and DCBLD2
DescriptionThree related datasets are included, all from immunoprecipitations of FLAG-tagged DCBLD1 and DCBLD2 (human) from 293 cells. Letters at the end of file names denote regions of each gel lave from highest (“A”) to lowest (“B”-X) molecular weight. File names containing “SILAC” are part of a dataset composed of 3 biological replicates (“T1”,”T2”,”T3” in file name) run of 10% or 15% gels (“10” or “15” in file name). DCBLD1 or DCBLD2 were immunoprecipitated from cells alone (light SILAC condition) or with Fyn/Abl (heavy SILAC condition). “Mock”, “Fyn” alone, or “Abl” alone in file names denote control immunoprecipitates, in which the light condition was a mock transfection and the heavy was either a mock or had Fyn/Abl expressed alone. Trypsin was used as the proteolytic enzyme. File names beginning with “Label_free” are part of datasets from LC-MS/MS analysis of DCBLD1 or DCBLD2 immunoprecipitates from 293 lysates. Prior to LC-MS/MS analysis, immunoprecipitates were digested with trypsin and GluC. Only DCBLD1 and DCBLD2 protein bands were analyzed via LC-MS/MS. File names containing “zebrafish” are part of datasets from LC-MS/MS analysis of DCBLD2 immunoprecipitates from 293 extracts that were then incubated with zebrafish extracts. These datasets include tryptic peptides from both human and zebrafish proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:10:26.221.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBryan Ballif
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationListphosphorylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-25 07:26:21ID requested
12020-08-06 22:59:43announced
22024-10-22 05:10:28announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra120.002163;
Schmoker AM, Weinert JL, Markwood JM, Albretsen KS, Lunde ML, Weir ME, Ebert AM, Hinkle KL, Ballif BA, FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family. Mol Cell Proteomics, 19(10):1586-1601(2020) [pubmed]
Keyword List
submitter keyword: CLCP1, DCBLD2, ESDN,DCBLD1
Contact List
Bryan Ballif
contact affiliationProfessor, Department of Biology, University of Vermont
contact emailbballif@uvm.edu
lab head
Bryan Ballif
contact affiliationDepartment of Biology
contact emailbballif@uvm.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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