PXD017723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fyn- and Abl-driven interactome and post-translational modifications of DCBLD1 and DCBLD2 |
Description | Three related datasets are included, all from immunoprecipitations of FLAG-tagged DCBLD1 and DCBLD2 (human) from 293 cells. Letters at the end of file names denote regions of each gel lave from highest (“A”) to lowest (“B”-X) molecular weight. File names containing “SILAC” are part of a dataset composed of 3 biological replicates (“T1”,”T2”,”T3” in file name) run of 10% or 15% gels (“10” or “15” in file name). DCBLD1 or DCBLD2 were immunoprecipitated from cells alone (light SILAC condition) or with Fyn/Abl (heavy SILAC condition). “Mock”, “Fyn” alone, or “Abl” alone in file names denote control immunoprecipitates, in which the light condition was a mock transfection and the heavy was either a mock or had Fyn/Abl expressed alone. Trypsin was used as the proteolytic enzyme. File names beginning with “Label_free” are part of datasets from LC-MS/MS analysis of DCBLD1 or DCBLD2 immunoprecipitates from 293 lysates. Prior to LC-MS/MS analysis, immunoprecipitates were digested with trypsin and GluC. Only DCBLD1 and DCBLD2 protein bands were analyzed via LC-MS/MS. File names containing “zebrafish” are part of datasets from LC-MS/MS analysis of DCBLD2 immunoprecipitates from 293 extracts that were then incubated with zebrafish extracts. These datasets include tryptic peptides from both human and zebrafish proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:10:26.221.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bryan Ballif |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | phosphorylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-25 07:26:21 | ID requested | |
1 | 2020-08-06 22:59:43 | announced | |
⏵ 2 | 2024-10-22 05:10:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra120.002163; |
Schmoker AM, Weinert JL, Markwood JM, Albretsen KS, Lunde ML, Weir ME, Ebert AM, Hinkle KL, Ballif BA, FYN and ABL Regulate the Interaction Networks of the DCBLD Receptor Family. Mol Cell Proteomics, 19(10):1586-1601(2020) [pubmed] |
Keyword List
submitter keyword: CLCP1, DCBLD2, ESDN,DCBLD1 |
Contact List
Bryan Ballif |
contact affiliation | Professor, Department of Biology, University of Vermont |
contact email | bballif@uvm.edu |
lab head | |
Bryan Ballif |
contact affiliation | Department of Biology |
contact email | bballif@uvm.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017723 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017723
- Label: PRIDE project
- Name: Fyn- and Abl-driven interactome and post-translational modifications of DCBLD1 and DCBLD2