PXD017716

PXD017716 is an original dataset announced via ProteomeXchange.

Title	Protective effect of Vigna unguiculata extract against aging and neurodegeneration
Description	Aging and age-related neurodegeneration are among the major challenges because of the progressive increase in the number of elder people in the wold population. Nutrition, which has important long-term consequences for health, is actually considered a means to prevent diseases and to reach a healthy aging. Here we investigate the role of Vigna unguiculata beans on senescence by using Saccharomyces cerevisiae and Drosophila melanogaster as model systems. Aqueous extract, mainly containing starch, proteins and amino acids, extends chronological lifespan in yeast cells, showing a remarkable synergistic effect in combination with caloric restriction. The extension of yeast longevity requires both the anti-aging Snf1/AMPK and the pro-aging Ras2/PKA pathways. A significant marked increase of lifespan was observed also in fruit flies supplemented with the V. unguiculata extract, which is accompanied by the increased expression of FOXO, NOTCH, SIRT1 and heme oxygenase (HO) genes, already known to be required for the extension of fruit fly longevity. α-synuclein forms toxic intracellular protein inclusions in Parkinson’s disease (PD) and actually preventing α-synuclein self-assembly has become one of the most promising approaches for the treatment of this neurodegenerative disorder. Here, we report that in vitro aggregation of -synuclein, as well as its toxicity in yeast and in neuroblastoma cells, are strongly decreased in the presence of bean extract. In addition, in a Caenorhabditis elegans model of PD that expresses α-synuclein, Vigna unguiculate extract substantially reduces the number of the age-dependent degeneration of the cephalic dopaminergic neurons. Overall, our data support the role of Vigna unguiculata beans as a functional food, worth to be further explored in order to develop lead molecules for therapeutic intervention in age-related disorders.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:36.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simona Nonnis
SpeciesList	scientific name: 'Phaseolus vulgaris' phytoplasma; NCBI TaxID: 188912;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-25 06:32:01	ID requested
1	2021-09-08 13:45:41	announced
⏵ 2	2024-10-22 05:26:38	announced	2024-10-22: Updated project metadata.

Tripodi F, Lombardi L, Guzzetti L, Panzeri D, Milanesi R, Leri M, Bucciantini M, Angeloni C, Beghelli D, Hrelia S, Onorato G, Di Schiavi E, Falletta E, Nonnis S, Tedeschi G, Labra M, Coccetti P, extract against aging and neurodegeneration. Aging (Albany NY), 12(19):19785-19808(2020) [pubmed]

10.18632/aging.104069;

submitter keyword: nutrition, Parkinson’s disease (PD),proteomics, functional food, Saccharomyces cerevisiae, nano LC-MS/MS, Caenorhabditis elegans, human -synuclein, Drosophila melanogaster

Simona Nonnis
contact affiliation	Department of Veterinary Medicine, University of Milan
contact email	simona.nonnis@unimi.it
lab head
Simona Nonnis
contact affiliation	University of Milan
contact email	simona.nonnis@unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD017716

PRIDE project URI

• PRIDE
  1. PXD017716
     1. Label: PRIDE project
     2. Name: Protective effect of Vigna unguiculata extract against aging and neurodegeneration