PXD017695

PXD017695 is an original dataset announced via ProteomeXchange.

Title	In-cell architecture of an active transcription-translation complex
Description	Structural biology performed inside cells can capture molecular machines in action within their native context. Here we develop an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae. We combine whole-cell crosslinking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at sub-nanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpoint NusA at the interface between the ribosome and a NusG-bound elongating RNAP, and propose it could mediate transcription-translation coupling. Transcription inhibition stalls and rearranges the expressome, whereas translation inhibition dissociates it, demonstrating that the elongating expressome architecture requires active translation and transcription within the cell.
HostingRepository	PRIDE
AnnounceDate	2020-07-30
AnnouncementXML	Submission_2020-07-29_22:16:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis O'Reilly
SpeciesList	scientific name: Mycoplasma pneumoniae M129; NCBI TaxID: 272634;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-24 00:10:15	ID requested
⏵ 1	2020-07-29 22:16:01	announced

Dataset with its publication pending

submitter keyword: Crosslinking-MS, CX-MS, CLMS, XL-MS, Mycoplasma Pneumoniae, DSS

Juri Rappsilber
contact affiliation	Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany Wellcome Centre for Cell Biology, University of Edinburgh, Max Born Crescent, Edinburgh, EH9 3BF, UK
contact email	juri.rappsilber@tu-berlin.de
lab head
Francis O'Reilly
contact affiliation	TU Berlin
contact email	oreilly@tu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017695

PRIDE project URI

• PRIDE
  1. PXD017695
     1. Label: PRIDE project
     2. Name: In-cell architecture of an active transcription-translation complex