PXD017693-1
PXD017693 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Isopeptides Between Human Tissue Transglutaminase and Wheat, Rye, and Barley Gluten Peptides |
Description | Celiac disease (CD) is a chronic immune-mediated enteropathy of the small intestine, which is triggered by the ingestion of storage proteins (gluten) from wheat, rye, and barley in genetically predisposed individuals. Human tissue transglutaminase (TG2) plays a central role in the pathogenesis of CD, because it is responsible for specific gluten peptide deamidation and covalent crosslinking between glutamine and lysine, resulting in the formation of Nε-(γ-glutamyl)-lysine isopeptide bonds. The resulting TG2-gluten peptide complexes are assumed to cause the secretion of anti-TG2-autoantibodies, but the underlying mechanisms are only partly known. To gain more insight into the structures of these complexes, the aim of our study was to identify TG2-gluten isopeptides. We applied a reciprocal proteomics strategy including discovery-driven as well as targeted proteomics to complex wheat, rye and barley gluten hydrolysates that had been incubated with TG2. In total, we detected 29 TG2-gluten isopeptides, involving seven selected TG2 lysine residues (K205, K265, K429, K468, K590, K600, K677). Several gluten peptides carried known B-cell epitopes and/or T-cell epitopes, either intact 9-mer core regions or partial sequences, as well as sequences bearing striking similarities to already known epitopes. These novel insights into the molecular structures of TG2-gluten peptide complexes may help clarify their physiological relevance in the initiation of CD autoimmunity and the role of anti-TG2 autoantibodies |
HostingRepository | PanoramaPublic |
AnnounceDate | 2020-08-27 |
AnnouncementXML | Submission_2020-08-27_13:19:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | supported by repository but incomplete data and/or metadata |
PrimarySubmitter | Barbara Lexhaller |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Hordeum vulgare; NCBI TaxID: 4513; scientific name: Triticum aestivum; NCBI TaxID: 4565; scientific name: Secale cereale; NCBI TaxID: 4550; |
ModificationList | Deamidated; Label:13C(6)15N(2) |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-02-23 17:30:22 | ID requested | |
⏵ 1 | 2020-08-27 13:19:20 | announced |
Publication List
Lexhaller B, Ludwig C, Scherf KA, Identification of Isopeptides Between Human Tissue Transglutaminase and Wheat, Rye, and Barley Gluten Peptides. Sci Rep, 10(1):7426(2020) [pubmed] |
Keyword List
submitter keyword: celiac disease, gluten, isopeptides, mass spectrometry, transglutaminase |
Contact List
Katharina Scherf | |
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contact affiliation | Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany |
contact email | k.scherf.leibniz-lsb@tum.de |
lab head | |
Barbara Lexhaller | |
contact affiliation | Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany |
contact email | b.lexhaller.leibniz-lsb@tum.de |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |