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PXD017649

PXD017649 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
DescriptionGeneration of oligodendrocytes (OLs) is a sophisticated multistep process, mechanistic underpinnings of which are not fully understood and demand further investigation. To systematically profile proteome dynamics during human embryonic stem cell (hESC) differentiation into OLs, we applied in-depth quantitative proteomics at different developmental stages and monitored changes in protein abundance using a multiplexed tandem mass tag (TMT) based proteomics approach. Findings: Our proteome data provided a comprehensive protein expression profile that highlighted specific expression clusters based on the protein abundances over the course of human OL lineage differentiation. The proteome profile of OL lineage cells revealed 378 proteins that were specifically up-regulated only in one differentiation stage. In addition, comparative pairwise analysis of differentiation stages demonstrated that abundances of 352 proteins differentially changed between consecutive differentiation time points. Our results highlighted the eminence of the planar cell polarity (PCP) signaling and autophagy (particularly macroautophagy) in the progression of OL lineage differentiation
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:12:25.157.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYunqi Wu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-20 01:21:17ID requested
12020-09-16 07:16:08announced
22020-11-03 03:43:20announced2020-11-03: Updated publication reference for PubMed record(s): 33128372.
32024-10-22 05:12:29announced2024-10-22: Updated project metadata.
Publication List
Pooyan P, Karamzadeh R, Mirzaei M, Meyfour A, Amirkhan A, Wu Y, Gupta V, Baharvand H, Javan M, Salekdeh GH, The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways. Gigascience, 9(11):(2020) [pubmed]
10.1093/gigascience/giaa116;
Keyword List
submitter keyword: human embryonic stem cell
neural stem cell
progenitor cell
oligodendrocyte
Wnt signaling
autophagy
quantitative proteomics
multiplexed tandem mass tag
Contact List
Ghasem Hosseini Salekdeh
contact affiliationDepartment of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
contact emailHosseini.salekdeh@mq.edu.au
lab head
Yunqi Wu
contact affiliationMacquarie University
contact emailyunqi.wu@mq.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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