PXD017649 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways |
Description | Generation of oligodendrocytes (OLs) is a sophisticated multistep process, mechanistic underpinnings of which are not fully understood and demand further investigation. To systematically profile proteome dynamics during human embryonic stem cell (hESC) differentiation into OLs, we applied in-depth quantitative proteomics at different developmental stages and monitored changes in protein abundance using a multiplexed tandem mass tag (TMT) based proteomics approach. Findings: Our proteome data provided a comprehensive protein expression profile that highlighted specific expression clusters based on the protein abundances over the course of human OL lineage differentiation. The proteome profile of OL lineage cells revealed 378 proteins that were specifically up-regulated only in one differentiation stage. In addition, comparative pairwise analysis of differentiation stages demonstrated that abundances of 352 proteins differentially changed between consecutive differentiation time points. Our results highlighted the eminence of the planar cell polarity (PCP) signaling and autophagy (particularly macroautophagy) in the progression of OL lineage differentiation |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:12:25.157.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yunqi Wu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-20 01:21:17 | ID requested | |
1 | 2020-09-16 07:16:08 | announced | |
2 | 2020-11-03 03:43:20 | announced | 2020-11-03: Updated publication reference for PubMed record(s): 33128372. |
⏵ 3 | 2024-10-22 05:12:29 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pooyan P, Karamzadeh R, Mirzaei M, Meyfour A, Amirkhan A, Wu Y, Gupta V, Baharvand H, Javan M, Salekdeh GH, The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways. Gigascience, 9(11):(2020) [pubmed] |
10.1093/gigascience/giaa116; |
Keyword List
submitter keyword: human embryonic stem cell |
neural stem cell |
progenitor cell |
oligodendrocyte |
Wnt signaling |
autophagy |
quantitative proteomics |
multiplexed tandem mass tag |
Contact List
Ghasem Hosseini Salekdeh |
contact affiliation | Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran |
contact email | Hosseini.salekdeh@mq.edu.au |
lab head | |
Yunqi Wu |
contact affiliation | Macquarie University |
contact email | yunqi.wu@mq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017649
- Label: PRIDE project
- Name: The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways