PXD017641

PXD017641 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomics of an NSD2-PWWP1 Chemical Probe
Description	Here we use competitive chemoproteomics pulldowns followed by label-free quantitative LC-MS/MS to assess target engagement and selectivity profiles of UNC6934 and UNC7145, a chemical probe targeting the PWWP1 domain of NSD2 and its negative control counterpart, respectively. To this end, we used a biotinylated probe derivative (UNC7096) for streptavidin pulldowns from KMS-11 multiple myeloma cell lysates, including in the context of UNC7145 or UNC6934 competition.
HostingRepository	PRIDE
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2021-08-06_03:03:33.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suzanne Ackloo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-19 23:05:56	ID requested
⏵ 1	2021-08-06 03:03:33	announced

Dataset with its publication pending

submitter keyword: chemoproteomics, epigenetics, chemical probe, histone methyltransferase

Cheryl H Arrowsmith
contact affiliation	Senior Scientist, Princess Margaret Cancer Center Medical Biophysics, University of Toronto
contact email	cheryl.arrowsmith@uhnresearch.ca
lab head
Suzanne Ackloo
contact affiliation	University of Toronto
contact email	suzanne.ackloo@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD017641

PRIDE project URI

• PRIDE
  1. PXD017641
     1. Label: PRIDE project
     2. Name: Chemoproteomics of an NSD2-PWWP1 Chemical Probe