PXD017620

PXD017620 is an original dataset announced via ProteomeXchange.

Title	Cross-linking mass spectrometry of yeast mitochondria grown on different carbon sources reveal Ndi1, Min8 and Pet9 associated with the electron transport chain
Description	Mitochondria derived from Saccharomyces cerevisiae grown on either a non-fermentable (glycerol) or a fermentable (glucose) carbon source were cross-linked with BS3. Additionally, by using a stable-isotope labelled quantitative cross-linking approach, we were able to quantify differences in protein-protein cross-links in mitochondria according to the growth condition. Furthermore, so far uncharacterized yeast proteins were put into biological context based on their cross-linking pattern.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-07-15_00:20:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Linden
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-19 07:42:40	ID requested
1	2020-04-27 00:27:12	announced
⏵ 2	2020-07-15 00:20:43	announced	2020-07-15: Updated publication reference for PubMed record(s): 32332106.

Linden A, Deckers M, Parfentev I, Pflanz R, Homberg B, Neumann P, Ficner R, Rehling P, Urlaub H, A Cross-linking Mass Spectrometry Approach Defines Protein Interactions in Yeast Mitochondria. Mol Cell Proteomics, 19(7):1161-1178(2020) [pubmed]

submitter keyword: cross-linking, mass spectrometry, XL-MS, quantitative, qXL-MS, mitochondria, yeast, Saccharomyces cerevisiae, carbon source

Henning Urlaub
contact affiliation	Max Planck Institute for Biophysical Chemistry, Göttingen, Germany and Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact email	Henning.Urlaub@mpibpc.mpg.de
lab head
Andreas Linden
contact affiliation	Max Planck Institute for Biophysical Chemistry and Clinical Chemistry, University Medical Center Göttingen
contact email	Andreas.Linden@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017620

PRIDE project URI

• PRIDE
  1. PXD017620
     1. Label: PRIDE project
     2. Name: Cross-linking mass spectrometry of yeast mitochondria grown on different carbon sources reveal Ndi1, Min8 and Pet9 associated with the electron transport chain