<<< Full experiment listing

PXD017619

PXD017619 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReal-time dynamics of Plasmodium Ndc80 reveals unusual modes of chromosome segregation during parasite proliferative stages
DescriptionEukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, has developed remarkable characteristic of nuclear division throughout its lifecycle to meet some peculiar and unique challenges of DNA replication and chromosome segregation. The parasite undergoes atypical endomitosis and endoreduplication with an intact nuclear membrane and intranuclear mitotic spindle. To understand diverse modes of cell division in Plasmodium we have studied the behaviour and composition of a key part of the mitotic apparatus, the outer kinetochore Ndc80 complex that attaches the centromere of chromosomes to the microtubules of the mitotic spindle. Using Ndc80 live-cell imaging in Plasmodium berghei we observe dynamic changes throughout proliferative life-stages including highly unusual kinetochore arrangements in sexual stages of the parasite. Furthermore, we identify a divergent Spc24 component of Ndc80 complex, previously thought to be missing, and completing a canonical, albeit unusual, Ndc80 complex structure. Altogether our studies reveal the kinetochore as an ideal starting point towards understanding non-canonical modes of chromosome segregation and cell division in Plasmodium.
HostingRepositoryPRIDE
AnnounceDate2020-06-09
AnnouncementXMLSubmission_2020-06-09_06:30:58.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD017619
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrew Bottrill
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListAmmonia-loss; Dehydrated; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-19 07:42:24ID requested
12020-06-09 06:30:59announced
Publication List
Zeeshan M, Pandey R, Ferguson DJP, Tromer EC, Markus R, Abel S, Brady D, Daniel E, Limenitakis R, Bottrill AR, Le Roch KG, Holder AA, Waller RF, Guttery DS, Tewari R, NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. J Cell Sci, 134(5):(2020) [pubmed]
Keyword List
submitter keyword: Malaria, Plasmodium, Ndc80, chromosome segregation, parasite, proliferation, Warwick-RTP
Contact List
Dr. Rita Tewari
contact affiliationSchool of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH
contact emailrita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliationUniversity of Warwick
contact emaila.r.bottrill@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017619
PRIDE project URI
Repository Record List
[ + ]