PXD017619 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Real-time dynamics of Plasmodium Ndc80 reveals unusual modes of chromosome segregation during parasite proliferative stages |
Description | Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, has developed remarkable characteristic of nuclear division throughout its lifecycle to meet some peculiar and unique challenges of DNA replication and chromosome segregation. The parasite undergoes atypical endomitosis and endoreduplication with an intact nuclear membrane and intranuclear mitotic spindle. To understand diverse modes of cell division in Plasmodium we have studied the behaviour and composition of a key part of the mitotic apparatus, the outer kinetochore Ndc80 complex that attaches the centromere of chromosomes to the microtubules of the mitotic spindle. Using Ndc80 live-cell imaging in Plasmodium berghei we observe dynamic changes throughout proliferative life-stages including highly unusual kinetochore arrangements in sexual stages of the parasite. Furthermore, we identify a divergent Spc24 component of Ndc80 complex, previously thought to be missing, and completing a canonical, albeit unusual, Ndc80 complex structure. Altogether our studies reveal the kinetochore as an ideal starting point towards understanding non-canonical modes of chromosome segregation and cell division in Plasmodium. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-09 |
AnnouncementXML | Submission_2020-06-09_06:30:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD017619 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrew Bottrill |
SpeciesList | scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823; |
ModificationList | Ammonia-loss; Dehydrated; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-02-19 07:42:24 | ID requested | |
⏵ 1 | 2020-06-09 06:30:59 | announced | |
Publication List
Zeeshan M, Pandey R, Ferguson DJP, Tromer EC, Markus R, Abel S, Brady D, Daniel E, Limenitakis R, Bottrill AR, Le Roch KG, Holder AA, Waller RF, Guttery DS, Tewari R, NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. J Cell Sci, 134(5):(2020) [pubmed] |
Keyword List
submitter keyword: Malaria, Plasmodium, Ndc80, chromosome segregation, parasite, proliferation, Warwick-RTP |
Contact List
Dr. Rita Tewari |
contact affiliation | School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, NG7 2UH |
contact email | rita.tewari@nottingham.ac.uk |
lab head | |
Andrew Bottrill |
contact affiliation | University of Warwick |
contact email | a.r.bottrill@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017619
- Label: PRIDE project
- Name: Real-time dynamics of Plasmodium Ndc80 reveals unusual modes of chromosome segregation during parasite proliferative stages