PXD017616

PXD017616 is an original dataset announced via ProteomeXchange.

Title	Role of hormones regulate productive diapause queens in year-round mass rearing of Bombus terrestris
Description	Adult reproductive diapause is a powerful overwintering strategy for many continental insect species including bumblebees, which enables queens to survive several months through harsh winter conditions and then build new beehives in the following spring. There are few reports regarding the molecular regulatory mechanism of reproductive diapause in Bombus terrestris, which is an important pollinators of wild plants and crops, and our previous researches identified the conditions for reproductive diapause of year-round mass rearing. Here, we performed combined RNA sequencing transcriptomics and quantitative proteomic analyses in different development phases relate to reproductive diapause. According to the overall analysis, we found these differentially expressed proteins/genes act in the citrate cycle, insect hormone biosynthesis, insulin and mTOR signalling pathway. To get better sense of the reproductive diapause regulated mechanism, some genes regulated JH synthesis, insulin/ TOR signal pathway were detected, the BtRheb, BtTOR, BtVg and BtJHAMT had lower expression levels in diapause queens, and the JH III titers levels and some metabolic enzymes activities were significantly up-regulated in found post-diapause queens. After microinjected insulin-like peptides (ILPs) and JH analog (JHA), some indicators shows the significantly changes of hormones, cold tolerance substances, metabolic enzymes and reproduction. Along with other related researches, a reproductive diapause regulated model during B. terrestris year-round mass rearing process was establishment. This study contribute to a comprehensive view and the molecular regulate mechanism of productive diapause in eusocial insect.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:44.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yifan Zhai
SpeciesList	scientific name: Bombus terrestris (Buff-tailed bumblebee) (Apis terrestris); NCBI TaxID: 30195;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-19 05:32:36	ID requested
1	2021-04-05 23:12:34	announced
⏵ 2	2024-10-22 05:20:46	announced	2024-10-22: Updated project metadata.

Chen H, Wu G, Zhou H, Dai X, Steeghs NWF, Dong X, Zheng L, Zhai Y, Queens. J Proteome Res, 20(5):2240-2250(2021) [pubmed]

10.1021/acs.jproteome.0c00776;

submitter keyword: social insects, hormones,Reproductive diapause, TMT., RNA-seq

Yifan Zhai
contact affiliation	Institute of Plant Protection, Shandong Academy of Agricultural Sciences, P.R.China
contact email	zyifan@tom.com
lab head
Yifan Zhai
contact affiliation	Institute of Plant Protection, Shandong Academy of Agricultural Sciences
contact email	zyifan@tom.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017616
     1. Label: PRIDE project
     2. Name: Role of hormones regulate productive diapause queens in year-round mass rearing of Bombus terrestris