PXD017591

PXD017591 is an original dataset announced via ProteomeXchange.

Title	A novel model of Amyloid-beta pathology in C. elegans reveals critically vulnerable neurons as seeds of pathogenicity
Description	A novel model of amyloid-beta pathology in C. elegans has been generated which allows for substoichiometric fluorescent labeling of amyloid-beta species in living nematodes. By microscopy, FLIM, physiological and biochemical studies, the progress of neurodegeneration and general pathogenicity of amyloidogenic peptides is confirmed. Notably, a single set of neurons was shown to be most vulnerable to Amyloid-beta overexpression and putatively act as a seed for systemic pathogeny. A neuronal version of the amyloid-beta reporter strain was used to perform proteomics analysis from worm lysates after enrichment by immunoprecipitation, using anti-amyloid-beta antibodies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:24.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuel Iburg
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-18 06:31:40	ID requested
1	2020-09-18 01:19:23	announced
⏵ 2	2024-10-22 05:12:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Amyloid beta

neurodegeneration

protein homeostasis

C. elegans

Janine Kirstein
contact affiliation	University of Bremen, Faculty 2 Biology / Chemistry
contact email	kirstein@uni-bremen.de
lab head
Manuel Iburg
contact affiliation	FMP Berlin
contact email	manuel_iburg@gmx.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017591

PRIDE project URI

• PRIDE
  1. PXD017591
     1. Label: PRIDE project
     2. Name: A novel model of Amyloid-beta pathology in C. elegans reveals critically vulnerable neurons as seeds of pathogenicity