PXD017585

PXD017585 is an original dataset announced via ProteomeXchange.

Title	MicroRNA-host gene lncRNA regulates the cell cycle re-entry post cellular quiescence
Description	Cellular quiescence is coupled with cellular development, tissue homeostasis, and cancer progression. Both quiescence and cell cycle re-entry are controlled by active and precise regulation of gene expression. However, the roles of long noncoding RNAs (lncRNAs) during these processes remain to be elucidated. By performing a genome-wide transcriptome analyses, we identify thousands of differentially expressed lncRNAs, including ~30 of the less-characterized class of microRNA-host-gene lncRNAs (lnc-MIRHGs), during cellular quiescence and during serum-stimulation in human diploid cells. We observe that the mature MIR222HG display serum-stimulated induction due to enhanced pre-RNA splicing. Serum-stimulated binding of the pre-mRNA splicing factor SRSF1 to a micro-exon, which partially overlaps with the primary miR-222 precursor, facilitates enhanced MIR222HG splicing. In serum-stimulated cells, SRSF1 negatively regulates the Drosha/DGCR8-catalyzed cleavage of pri-miR-222, thereby increasing the cellular pool of the mature MIR222HG. Further, loss-of-function studies indicate that the mature MIR222HG facilitates the serum-stimulated cell cycle re-entry in a microRNA-independent manner. Mechanistically, MIR222HG, along with ILF3/2 complex, forms RNA:RNA duplex with DNM3OS lncRNA, thereby promoting DNM3OS stability. The current study identifies a mechanism in which the interplay between splicing versus microprocessor complex dictates the serum-induced expression of lnc-MIRHG MIR222HG for efficient cell cycle re-entry.
HostingRepository	PRIDE
AnnounceDate	2020-09-07
AnnouncementXML	Submission_2020-09-06_23:50:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017585
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lisa Jenkins
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-18 05:35:41	ID requested
⏵ 1	2020-09-06 23:50:29	announced

Sun Q, Hao Q, Lin YC, Song YJ, Bangru S, Arif W, Tripathi V, Zhang Y, Cho JH, Freier SM, Jenkins LM, Ma J, Yoon JH, Kalsotra A, Lal A, Prasanth SG, Prasanth KV, for efficient cell cycle reentry. RNA, 26(11):1603-1620(2020) [pubmed]

submitter keyword: miR-222

quiescence

cell cycle

lncRNA

Lisa M. Jenkins
contact affiliation	Center for Cancer Research, National Cancer Institute, National Institutes of Health
contact email	lisa.jenkins@nih.gov
lab head
Lisa Jenkins
contact affiliation	National Cancer Institute, NIH
contact email	lisa.jenkins@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD017585

PRIDE project URI

• PRIDE
  1. PXD017585
     1. Label: PRIDE project
     2. Name: MicroRNA-host gene lncRNA regulates the cell cycle re-entry post cellular quiescence