PXD017582

PXD017582 is an original dataset announced via ProteomeXchange.

Title	Proteomic studies of Escherichia coli CFT073 and MG1655
Description	Here we dissected a regulatory network directed by the conserved iron homeostasis regulator, Ferric Uptake Regulator (Fur), in uropathogenic E. coli strain CFT073. Comparing anaerobic genome-scale Fur DNA binding, with Fur dependent transcript expression and protein levels of the uropathogen to that of commensal E. coli K-12 strain MG1655, showed that the Fur regulon of the core genome is conserved but also includes genes within the pathogenicity/genetic islands. Unexpectedly, regulons indicative of amino acid limitation and the general stress response were also indirectly activated in the uropathogen fur mutant, suggesting that induction of the Fur regulon increases amino acid demand. Using RpoS levels as a proxy, addition of amino acids mitigated the stress. In addition, iron chelation increased RpoS to the same levels as in the fur mutant. The increased amino acid demand of the fur mutant or iron chelated cells was exacerbated by aerobic conditions, which could be partly explained by the O 2 -dependent synthesis of the siderophore aerobactin, encoded within a pathogenicity island. Taken together, this data suggest in the iron-poor environment of the urinary tract, amino acid availability could play a role in the proliferation of this uropathogen, particularly if there is sufficient O 2 to produce aerobactin.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:38:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evgenia Shishkova
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-18 02:44:18	ID requested
⏵ 1	2020-05-26 16:38:08	announced

Banerjee R, Weisenhorn E, Schwartz KJ, Myers KS, Glasner JD, Perna NT, Coon JJ, Welch RA, Kiley PJ, Tailoring a Global Iron Regulon to a Uropathogen. mBio, 11(2):(2020) [pubmed]

submitter keyword: Fur, RyhB, iron regulation, CFT073, UPEC, ppGpp, metabolic adaptation, Sigma S

Joshua J Coon
contact affiliation	Professor of Biomolecular Chemistry at the University of Wisconsin - Madison
contact email	jcoon@chem.wisc.edu
lab head
Evgenia Shishkova
contact affiliation	University of Wisconsin - Madison
contact email	shishkova@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017582

PRIDE project URI

• PRIDE
  1. PXD017582
     1. Label: PRIDE project
     2. Name: Proteomic studies of Escherichia coli CFT073 and MG1655