PXD017545

PXD017545 is an original dataset announced via ProteomeXchange.

Title	N-linked glycosylation of recombinant soluble ectodomain of HKU1 haemagglutinin esterase
Description	Coronaviruses (CoVs) encompass many human pathogens such as HKU-1, OC43, NL63, SARS, MERS and, most recently, nCoV-2019. The spike (S) protein of CoVs has received much attention for its role in host tropism and immunity, but it is becoming increasingly clear that the haemagglutinin esterase (HE) also plays an important role in host adaptation by determining host receptor (sialic acid) specificity. We determined the structure of HKU1 HE by cryo electron microscopy and mapped site-specific N-linked glycosylation by LC-MS/MS of glycopeptides using electron transfer high-energy collision dissociation.
HostingRepository	PRIDE
AnnounceDate	2020-07-24
AnnouncementXML	Submission_2020-10-19_01:31:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joost Snijder
SpeciesList	scientific name: Human coronavirus HKU1; NCBI TaxID: 290028;
ModificationList	N-glycosylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-17 06:14:37	ID requested
1	2020-03-29 22:59:43	announced
⏵ 2	2020-10-19 01:31:39	announced	2020-10-19: Updated publication reference for PubMed record(s): 32938911.

Hurdiss DL, Drulyte I, Lang Y, Shamorkina TM, Pronker MF, van Kuppeveld FJM, Snijder J, de Groot RJ, Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans. Nat Commun, 11(1):4646(2020) [pubmed]

submitter keyword: glycosylation, coronavirus, structure, EThcD

Joost Snijder
contact affiliation	Utrecht University
contact email	j.snijder@uu.nl
lab head
Joost Snijder
contact affiliation	Utrecht University
contact email	joost.snijder@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD017545

PRIDE project URI

• PRIDE
  1. PXD017545
     1. Label: PRIDE project
     2. Name: N-linked glycosylation of recombinant soluble ectodomain of HKU1 haemagglutinin esterase