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PXD017537

PXD017537 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic analysis of seminal plasma, sperm membrane proteins and seminal extracellular vesicles suggests vesicular mechanisms aid in the removal and addition of proteins to the ram sperm membrane.
DescriptionQuantitative proteomic studies are contributing greatly to our understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the male reproductive tract. The aim of this study was to identify EV proteins isolated from ram seminal plasma. High-speed centrifugation using Optiprep sucrose gradients was used to purify two EV populations from seminal plasma. Liquid chromatography and tandem mass spectrometry (LC-MS/MS) with quantitative SWATH was used to identify proteins enriched in the extracellular vesicle preparation (e.g. EDIL3) and those that were co-isolated with vesicular preparations due to their abundance or adhesive nature (e.g. BSPs). Ram sperm plasma membrane proteins were also isolated using nitrogen cavitation and identified with LC-MS/MS to better understand the interplay of proteins between the sperm membrane and extracellular environment. The categorisation of proteins enriched in the EV population (P<0.05, 2-fold increase compared to whole seminal plasma) according to their function revealed three main groupings: vesicle biogenesis, metabolism and membrane adhesion and remodelling. The latter group contained many reproduction-specific proteins that show demonstrable links to sperm fertility. Many of these membrane-bound proteins show testicular expression and are shed from the sperm surface during epididymal maturation (e.g. TEX101, LY6K). Their association with seminal EVs suggests that EVs may not only deliver protein cargo to spermatozoa but also assist in the removal of proteins from the sperm membrane.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:03:21.233.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTaylor Pini
SpeciesList scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-17 03:04:53ID requested
12020-05-07 22:21:46announced
22020-06-09 23:30:55announced2020-06-10: Updated publication reference for PubMed record(s): 32383290.
32024-10-22 05:03:28announced2024-10-22: Updated project metadata.
Publication List
Leahy T, Rickard JP, Pini T, Gadella BM, de Graaf SP, Quantitative Proteomic Analysis of Seminal Plasma, Sperm Membrane Proteins, and Seminal Extracellular Vesicles Suggests Vesicular Mechanisms Aid in the Removal and Addition of Proteins to the Ram Sperm Membrane. Proteomics, 20(12):e1900289(2020) [pubmed]
10.1002/pmic.201900289;
Keyword List
submitter keyword: prostasomes, microvesicles, EDIL3,epididysomes, sperm surface
Contact List
Taylor Pini
contact affiliationColorado Center for Reproductive Medicine
contact emailtpini@flcolo.com
lab head
Taylor Pini
contact affiliationUniversity of Sydney
contact emailtpini@flcolo.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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