PXD017533

PXD017533 is an original dataset announced via ProteomeXchange.

Title	Hunting monolignol transporter(s): membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce
Description	Monolignol transport during lignification is a partially solved puzzle: both the mechanism(s) and the transported form of monolignols are unknown in developing xylem of trees. We tested a hypothesis of an active, plasma membrane (PM)-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared of developing xylem, phloem, and lignin-forming, tissue-cultured cells of Norway spruce (Picea abies L. Karst.), as well as of a control material, non-lignifying tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested this transport being over the tonoplast. Based on similar inhibitor assays, lignin-forming, tissue42 cultured cells of spruce had coniferin transport putatively localizing on PM. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis of membrane proteins isolated from spruce developing xylem, phloem and tissue-cultured cells revealed multiple transporters. These were compared to a transporter gene set that was gained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for the ABC-transporter-mediated monolignol transport but point to secondary active transporters (such as MFS- or MATE-transporters). In contrast, proteomic and co-expression analyses suggest a role for ABC-transporters and MFS-transporters.
HostingRepository	PRIDE
AnnounceDate	2020-08-14
AnnouncementXML	Submission_2020-08-14_02:54:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joakim Bygdell
SpeciesList	scientific name: Picea abies; NCBI TaxID: 3329;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt G2-S HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-17 00:28:26	ID requested
⏵ 1	2020-08-14 02:55:00	announced

V, ä, is, ä, nen E, Takahashi J, Obudulu O, Bygdell J, Karhunen P, Blokhina O, Laitinen T, Teeri TH, Wingsle G, Fagerstedt KV, K, ä, rk, ö, nen A, Hunting monolignol transporters: membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce. J Exp Bot, 71(20):6379-6395(2020) [pubmed]

submitter keyword: Norway Spruce, Picea abies, monolignol transport, lignin biosynthesis, plasma membrane, transporter proteins

Anna Kärkönen
contact affiliation	Viikki Plant Science Centre, Department of Agricoultural Sciences, University of Helsinki, Finland Natural Resources Institute Finland (Luke), Production Systems, Plant Genetics, Helsinki Finland
contact email	anna.karkonen@luke.fi
lab head
Joakim Bygdell
contact affiliation	Computational Life Science Cluster (CLiC), Deptartment of Chemistry, Umeå University
contact email	joakim.bygdell@umu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017533
     1. Label: PRIDE project
     2. Name: Hunting monolignol transporter(s): membrane proteomics and biochemical transport assays with membrane vesicles of Norway spruce