PXD017529

PXD017529 is an original dataset announced via ProteomeXchange.

Title	HDL Quality Features Revealed by Proteome-Lipidome Connectivity are Associated with Atherosclerotic Disease
Description	The “quality” rather than “quantity” of lipoprotein particles is more relevant to cardiovascular diseases (CVD), since lipoprotein particles are highly heterogeneous. However, the targeted separation, detection, and exploration of the heterogeneous on omic-scale for lipoprotein remain challenging and rarely touched. Here, we established a high-resolution proteomics and lipidomics (HiPL) method. Briefly, the lipoprotein particles were separated into nine fractions (Lipo-HiPL) using anion-exchange chromatography (AEC), in which HDL, LDL, and VLDL were fractionated into three more fractions respectively. Furthermore, the HDL subclasses were delicately purified within nine fractions (HDL-HiPL), covering diverse protein-lipid heterogeneity. Integration of proteomic and lipidomic analysis revealed proteome-lipidome connectivity (PLC) in lipoprotein and HDL particles, which highly correlated with the atherosclerotic phenotype in rabbit model. Importantly, the lipoprotein “quality” revealed by PLC was found to be related more closely to clinical status in acute coronary syndrome patients than direct proteome and lipidome quantification. Thus, proteome-lipidome connectivity (PLC) as novel HDL “quality” features could be highly associated with atherosclerotic cardiovascular diseases.
HostingRepository	iProX
AnnounceDate	2020-02-15
AnnouncementXML	Submission_2023-08-27_21:00:17.106.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingrun Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-15 03:17:12	ID requested
⏵ 1	2023-08-27 21:00:19	announced

Wang D, Yu B, Li Q, Guo Y, Koike T, Koike Y, Wu Q, Zhang J, Mao L, Tang X, Sun L, Lin X, Wu J, Chen YE, Peng D, Zeng R, lipidome connectivity are associated with atherosclerotic disease. J Mol Cell Biol, 14(3):(2022) [pubmed]

submitter keyword: HDL, high-resolution, proteomics, lipidomics, atherosclerotic diseases

Rong Zeng
contact affiliation	Institute of Biochemistry and Cell Biology
contact email	zr@sibcb.ac.cn
lab head
Qingrun Li
contact affiliation	Shanghai Institutes for Biological Sciences, CAS
contact email	qrli@sibs.ac.cn
dataset submitter

iProX dataset URI