PXD017528

PXD017528 is an original dataset announced via ProteomeXchange.

Title	Chloroplast proteomics of wheat under drought
Description	Perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. Exogenous application of 5-aminolevulinic acid (ALA) mitigates the damages caused by drought stress protecting plant growth and development, but the regulatory mechanism behind was still obscure. Wheat seedlings were drought-treated, and the iTRAQ based proteomics approach was employed to assess the difference in chloroplast protein expression regulation caused by exogenous ALA. A total of 9499 peptides were identified with ≤ 0.01 FDR, which could be classified into 2442 protein groups. Moreover, the expressions of 87 chloroplast proteins were changed by drought stress alone compared to drought-free control, while 469 were changed by exogenous ALA application under drought stress compared to drought stress alone. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results suggested that ALA pre-treatment adjusted some biological pathways to enhancing the drought resistance of chloroplast, such as metabolic pathways, photosynthesis, and ribosome. Furthermore, the drought promoted H2O2 accumulation and O2- production in chloroplast were alleviated by exogenous pre-treatment of ALA, while the peroxidase (POD) and glutathione peroxidase (GPX) activities were up-regulated, which were coincident with the chloroplast proteomics data. We suggested that ALA promoted the reactive oxygen species (ROS) scavenging in chloroplast by regulating enzymatic processes. Our results extend the understanding of the mechanisms employed by exogenous ALA to defend against drought stress in wheat according to the chloroplast proteomics.
HostingRepository	iProX
AnnounceDate	2018-12-10
AnnouncementXML	Submission_2023-03-01_23:10:42.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuexia Wang
SpeciesList	scientific name: Triticum aestivum; NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 21:29:51	ID requested
1	2020-02-14 21:32:33	announced
⏵ 2	2023-03-01 23:10:45	announced	2023-03-02: Update publication information.

Wang Y, Li X, Liu N, Wei S, Wang J, Qin F, Suo B, The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis. BMC Plant Biol, 20(1):96(2020) [pubmed]

submitter keyword: Chloroplast proteomics, wheat, drought, ALA

Yuexia Wang
contact affiliation	Henan Agricultural University
contact email	yxwang2100@126.com
lab head
Yuexia Wang
contact affiliation	Henan Agricultural University
contact email	yxwang2100@126.com
dataset submitter

iProX dataset URI