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PXD017526

PXD017526 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics on HeLa cell surface (iTraq)
DescriptionWhile several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a clathrin-independent cargo. We show that endophilin-A3 – but neither A1 nor A2 isoforms – functionally associates with CD166-containing early endocytic carriers and physically interacts with the cargo. Our data further demonstrates that the three endophilin-A isoforms control the uptake of distinct subsets of cargoes. In addition, we provide strong evidence that the construction of endocytic sites from which CD166 is taken up in an endophilin-A3-dependent manner is driven by extracellular galectin-8. Taken together, our data reveal the existence of a previously uncharacterized clathrin-independent endocytic modality, that modulates the abundance of CD166 at the cell surface, and regulates adhesive and migratory properties of cancer cells.
HostingRepositoryPRIDE
AnnounceDate2020-04-22
AnnouncementXMLSubmission_2020-04-22_03:22:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHenri-François Renard
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-14 08:55:16ID requested
12020-04-22 03:22:43announced
Publication List
Renard HF, Tyckaert F, Lo Giudice C, Hirsch T, Valades-Cruz CA, Lemaigre C, Shafaq-Zadah M, Wunder C, Wattiez R, Johannes L, van der Bruggen P, Alsteens D, Morsomme P, Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun, 11(1):1457(2020) [pubmed]
Keyword List
submitter keyword: HeLa, iTraq
Contact List
Pierre Morsomme
contact affiliationUniversité catholique de Louvain (UCLouvain) Louvain Institute of Biomolecular Science and Technology (LIBST) Croix du Sud, 4-5 bte L7.07.14 1348 Louvain-la-Neuve Belgium
contact emailpierre.morsomme@uclouvain.be
lab head
Henri-François Renard
contact affiliationUCLouvain
contact emailhenri-francois.renard@uclouvain.be
dataset submitter
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