PXD017526

PXD017526 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics on HeLa cell surface (iTraq)
Description	While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a clathrin-independent cargo. We show that endophilin-A3 – but neither A1 nor A2 isoforms – functionally associates with CD166-containing early endocytic carriers and physically interacts with the cargo. Our data further demonstrates that the three endophilin-A isoforms control the uptake of distinct subsets of cargoes. In addition, we provide strong evidence that the construction of endocytic sites from which CD166 is taken up in an endophilin-A3-dependent manner is driven by extracellular galectin-8. Taken together, our data reveal the existence of a previously uncharacterized clathrin-independent endocytic modality, that modulates the abundance of CD166 at the cell surface, and regulates adhesive and migratory properties of cancer cells.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-04-22_03:22:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henri-François Renard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-02-14 08:55:16	ID requested
⏵ 1	2020-04-22 03:22:43	announced

Renard HF, Tyckaert F, Lo Giudice C, Hirsch T, Valades-Cruz CA, Lemaigre C, Shafaq-Zadah M, Wunder C, Wattiez R, Johannes L, van der Bruggen P, Alsteens D, Morsomme P, Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun, 11(1):1457(2020) [pubmed]

submitter keyword: HeLa, iTraq

Pierre Morsomme
contact affiliation	Université catholique de Louvain (UCLouvain) Louvain Institute of Biomolecular Science and Technology (LIBST) Croix du Sud, 4-5 bte L7.07.14 1348 Louvain-la-Neuve Belgium
contact email	pierre.morsomme@uclouvain.be
lab head
Henri-François Renard
contact affiliation	UCLouvain
contact email	henri-francois.renard@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017526

PRIDE project URI

• PRIDE
  1. PXD017526
     1. Label: PRIDE project
     2. Name: Quantitative proteomics on HeLa cell surface (iTraq)